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Data and Publications
(A) Primary human airway epithelial cells were expanded in a submerged culture with PneumaCult™-Ex Medium (Catalog #05008) and labeled with Anti- Human p63 (deltaN) Antibody, Clone Poly6190, followed by a goat anti-rabbit IgG antibody, Alexa Fluor® 594. (B) DAPI counterstaining of the cells shown in figure (A). (C) Primary human airway epithelial cells cultured in PneumaCult™-ALI Medium (Catalog #05001) at the air-liquid interface were cryo-sectioned and labeled with Anti-Human p63 (deltaN) Antibody, Clone Poly6190 (red), followed by a goat anti-rabbit IgG antibody, Alexa Fluor® 594 (red), and antihuman NGF Receptor/p75NTR (CD271) antibody, followed by a donkey anti-mouse IgG antibody, Alexa Fluor® 488 (green). (D) Western blot analysis of non-transfected HEK293 cell lysates (negative control; lane 1) and p63 (deltaN)-transfected HEK293 cell lysates (lane 2). Lysates were resolved by electrophoresis, transferred to nitrocellulose, and probed with Anti-Human p63 (deltaN) Antibody, Clone Poly6190. Proteins were visualized using an HRP-conjugated donkey anti-rabbit IgG secondary antibody and chemiluminescence detection.