Anti-Human HLA-DR Antibody, Clone L243

Mouse monoclonal IgG2a antibody against human, rhesus, cynomolgus HLA-DR
From: 69 USD

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Mouse monoclonal IgG2a antibody against human, rhesus, cynomolgus HLA-DR
From: 69 USD

Overview

The L243 antibody reacts with human HLA-DR, the major histocompatibility complex (MHC) class II receptor and a member of the immunoglobulin protein superfamily. HLA-DR is a heterodimeric transmembrane glycoprotein comprising a 36-kDa α subunit associated non-covalently with a 27-kDa β subunit and is expressed on the surface of antigen-presenting cells such as B cells, activated T cells, monocytes, macrophages and dendritic cells, activated natural killer (NK) cells, and progenitor cells. Together with the CD3/T cell receptor (TCR) complex and CD4 molecules, HLA-DR mediates a critical function in presenting peptides generated from hydrolysis of exogenous antigens by antigen-presenting cells to CD4+T (helper) cells, thereby either suppressing or inducing an immune response to the peptides. Thus, HLA-DR is involved in graft-versus-host disease and several autoimmune conditions. The L243 antibody binds an extracellular, conformational, and nonpolymorphic epitope on the α chain that is expressed only when the αβ heterodimer is correctly folded, and binding is not dependent on peptide loading of HLA-DR. Binding of the antibody blocks the association of HLA-DR with TCRs and reportedly exerts cytotoxic effects on human cells. The L243 antibody does not cross-react with HLA-DQ or HLA-DP.
Subtype:
Primary Antibodies
Target Antigen:
HLA-DR
Alternative Names:
HLA-DRA, Major histocompatibility class II, Major histocompatibility class II DR alpha, MHC class II, MHC class II DR alpha, MGC117330, MLRW
Reactive Species:
African Green Monkey; Baboon; Common Marmoset; Cotton-Topped Tamarin; Cynomolgus; Pigtailed Macaque; Rhesus; Squirrel Monkey; Chimpanzee; Dog; Human
Conjugation:
APC-Cyanine7; APC; FITC; PE
Host Species:
Mouse
Cell Type:
B Cells; Macrophages; Monocytes; T Cells
Application:
Flow Cytometry; Immunohistochemistry
Area of Interest:
Immunology
Clone:
L243
Gene ID:
3122, 3123
Isotype:
IgG2a, kappa

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

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Workflow Stages

Data and Publications

Data

Figure showing flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human HLA-DR Antibody, Clone L243, APC or a mouse IgG2a, kappa APC isotype control antibody.

Figure 1. Data for Anti-Human HLA-DR Antibody, Clone L243, APC

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human HLA-DR Antibody, Clone L243, APC (filled histogram) or a mouse IgG2a, kappa APC isotype control antibody (solid line histogram). Viable lymphocytes were gated for analysis.

Figure showing flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human HLA-DR Antibody, Clone L243, APC-Cyanine7 or a mouse IgG2a, kappa APC-Cyanine7 isotype control antibody.

Figure 2. Data for Anti-Human HLA-DR Antibody, Clone L243, APC-Cy7

Flow cytometry analysis of human PBMCs labeled with Anti-Human HLA-DR Antibody, Clone L243, APC-Cyanine7 (filled histogram) or a mouse IgG2a, kappa APC-Cyanine7 isotype control antibody (solid line histogram). Viable lymphocytes were gated for analysis.

Figure showing flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human HLA-DR Antibody, Clone L243, FITC or a mouse IgG2a, kappa FITC isotype control antibody.

Figure 3. Data for Anti-Human HLA-DR Antibody, Clone L243, FITC

Flow cytometry analysis of human PBMCs labeled with Anti-Human HLA-DR Antibody, Clone L243, FITC (filled histogram) or a mouse IgG2a, kappa FITC isotype control antibody (solid line histogram). Viable lymphocytes were gated for analysis.

Figure showing flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human HLA-DR Antibody, Clone L243, PE or a mouse IgG2a, kappa PE isotype control antibody.

Figure 4. Data for Anti-Human HLA-DR Antibody, Clone L243, PE

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human HLA-DR Antibody, Clone L243, PE (filled histogram) or a mouse IgG2a, kappa PE isotype control antibody (solid line histogram). Viable lymphocytes were gated for analysis.

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.