Anti-Human CD9 Antibody, Clone HI9a

Mouse monoclonal antibody against human, rhesus, cynomolgus CD9, unconjugated
Anti-Human CD9 Antibody, Clone HI9a

Mouse monoclonal antibody against human, rhesus, cynomolgus CD9, unconjugated

100 µg
Catalog # 100-0138
294 USD

Overview

The HI9a antibody reacts with an extracellular epitope on human CD9, a 24 kDa single-chain type III transmembrane glycoprotein. CD9 is broadly expressed, including on the surface of monocytes, developing B cells, activated T cells, granulocytes, epithelial cells, endothelial cells, and platelets (mainly the α-granules) and is also a marker characteristic of extracellular vesicles.

CD9 belongs to the tetraspanin family of proteins, which contain four transmembrane domains, two extracellular loops, and short cytoplasmic N- and C-termini. It is involved in mediating several cellular processes, including angiogenesis, by promoting cell adhesion, migration, and signal transduction, and is involved in cell fusion processes linked to fertilization, osteoclastogenesis, and myogenesis.
Subtype
Primary Antibodies
Target Antigen
CD9
Alternative Names
DRAP-24
Reactive Species
African Green Monkey, Baboon, Capuchin Monkey, Cynomolgus, Rhesus, Chimpanzee, Human
Conjugation
Unconjugated
Host Species
Mouse
Cell Type
Other
Application
Flow Cytometry, Western Blotting
Area of Interest
Extracellular Vesicle Research
Clone
HI9a
Gene ID
928
Isotype
IgG1, kappa

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
Anti-Human CD9 Antibody, Clone HI9a
Catalog #
100-0138
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD9 Antibody, Clone HI9a
Catalog #
100-0138
Lot #
All
Language
English

Educational Materials (5)

Brochure
Isolate and Purify Extracellular Vesicles
Protocol
How to Characterize Extracellular Vesicles by Western Blotting
Wallchart
Mesenchymal Stromal Cell-Derived Exosomes: Biogenesis and Cargoes
Webinar
Decoding Intercellular Communication via Hepatocyte-Secreted Extracellular Vesicles
35:31
Decoding Intercellular Communication via Hepatocyte-Secreted Extracellular Vesicles
Webinar
Online Immunology Journal Club: Viral Exploitation of Extracellular Vesicles to Spread Infection
23:18
Online Immunology Journal Club: Viral Exploitation of Extracellular Vesicles to Spread Infection

Data and Publications

Data

Data showing western blot analysis of extracellular vesicles isolated from mesenchymal stromal cell-conditioned medium with Anti-Human CD9 Antibody, Clone HI9a and flow cytometry analysis of human peripheral blood mononuclear cells labeled with Anti-Human CD9 Antibody, Clone HI9a.

Figure 1. Analysis of Anti-Human CD9 Antibody, Clone HI9a with Western Blot and Flow Cytometry

Extracellular vesicles (EVs) were isolated from mesenchymal stromal cell (MSC)-conditioned medium using a 2 mL EV size exclusion chromatography column and analyzed by (A) western blot with Anti-Human CD9 Antibody, Clone HI9a. Lanes (left to right) were loaded with isolated fractions 9, 10, and 11, respectively. Further validation was performed by flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs; viable lymphocytes gated for analysis) stimulated with phorbol myristate acetate (PMA) and ionomycin and then (B) labeled with Anti-Human CD9 Antibody, Clone HI9a, followed by a goat anti-mouse IgG1 antibody, APC (filled histogram), or Anti-Dextran Antibody, Clone DX1 (Catalog #60026) as an isotype control, followed by a goat anti-mouse IgG1 antibody, APC (solid line histogram), or (C) labeled with Anti-Dextran Antibody, Clone DX1 (Catalog #60026) as an isotype control, followed by a goat anti-mouse IgG1 antibody, APC, or (D) labeled with Anti-Human CD9 Antibody, Clone HI9a, followed by a goat anti-mouse IgG1 antibody, APC.

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