Anti-Human CD9 Antibody, Clone HI9a
Mouse monoclonal antibody against human, rhesus, cynomolgus CD9, unconjugated
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Overview
CD9 belongs to the tetraspanin family of proteins, which contain four transmembrane domains, two extracellular loops, and short cytoplasmic N- and C-termini. It is involved in mediating several cellular processes, including angiogenesis, by promoting cell adhesion, migration, and signal transduction, and is involved in cell fusion processes linked to fertilization, osteoclastogenesis, and myogenesis.
Data Figures
Figure 1. Analysis of Anti-Human CD9 Antibody, Clone HI9a with Western Blot and Flow Cytometry
Extracellular vesicles (EVs) were isolated from mesenchymal stromal cell (MSC)-conditioned medium using a 2 mL EV size exclusion chromatography column and analyzed by (A) western blot with Anti-Human CD9 Antibody, Clone HI9a. Lanes (left to right) were loaded with isolated fractions 9, 10, and 11, respectively. Further validation was performed by flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs; viable lymphocytes gated for analysis) stimulated with phorbol myristate acetate (PMA) and ionomycin and then (B) labeled with Anti-Human CD9 Antibody, Clone HI9a, followed by a goat anti-mouse IgG1 antibody, APC (filled histogram), or Anti-Dextran Antibody, Clone DX1 (Catalog #60026) as an isotype control, followed by a goat anti-mouse IgG1 antibody, APC (solid line histogram), or (C) labeled with Anti-Dextran Antibody, Clone DX1 (Catalog #60026) as an isotype control, followed by a goat anti-mouse IgG1 antibody, APC, or (D) labeled with Anti-Human CD9 Antibody, Clone HI9a, followed by a goat anti-mouse IgG1 antibody, APC.
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Resources and Publications
Educational Materials (4)
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Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
