Make more informed purchasing decisions with our new product availability and delivery estimate feature, now available on all product pages, in your cart, and during checkout.
Sign In
New to STEMCELL?
Register for an account to quickly and easily purchase products online and for one-click access to all educational content.
Thank you for your interest in this product.
Please provide us with your contact information and your local representative
will contact you with a customized quote. Where appropriate, they can also assist you with a(n):
Estimated delivery time for your area
Product sample or exclusive offer
In-lab demonstration
By submitting this form, you are providing your consent to STEMCELL Technologies Canada Inc. and its subsidiaries and affiliates (“STEMCELL”) to collect and use your information, and send you newsletters and emails in accordance with our privacy policy. Please contact us with any questions that you may have. You can unsubscribe or change your email preferences at any time.
The OKT9 antibody reacts with CD71 (transferrin receptor), an ~180 - 190 kDa disulfide-bonded homodimer and type 2 transmembrane glycoprotein expressed at high levels on a broad range of actively proliferating cells, as well as reticulocytes, monocytes, macrophages and marrow stromal cells. Surface levels are low on resting leukocytes but are upregulated on lymphocytes, monocytes and macrophages following antigen or mitogen stimulation. Expression is lost during differentiation of reticulocytes into mature erythrocytes. CD71 mediates uptake of transferrin–iron complexes through receptor-mediated endocytosis and recycling of the apotransferrin–receptor complex to the cell surface. CD71 has been employed as a marker for evaluating erythroid precursors and for assessing disorders such as erythroid leukemia and myelodysplastic syndrome. It has also served as a target for drug delivery. The OKT9 antibody binds an epitope that reportedly overlaps with that of antibody clone CY1G4 but is distinct from the ligand binding site for transferrin.
(A) Flow cytometry analysis of CD3+ cells (T cells) isolated from human peripheral blood mononuclear cells (PBMCs), then stimulated by incubation with anti-CD3 and anti-CD28 antibodies. Cells were labeled with Anti-Human CD71 (Transferrin Receptor) Antibody, Clone OKT9, followed by a rat anti-mouse IgG1 antibody, PE (filled histogram), or a mouse IgG1, kappa isotype control antibody followed by a rat anti-mouse IgG1 antibody, PE (solid line histogram). (B) Flow cytometry analysis of CD3+ cells isolated from human PBMCs, then stimulated by incubation with anti-CD3 and anti-CD28 antibodies. Cells were labeled with Anti-Human CD71 (Transferrin Receptor) Antibody, Clone OKT9, which had been pre-incubated for 5 min with a rat anti-mouse IgG1 antibody, PE (filled histogram), or a mouse IgG1, kappa isotype control antibody, which had been pre-incubated for 5 min with a rat anti-mouse IgG1 antibody, PE (solid line histogram). Pre-incubating the primary and secondary antibodies helped minimize background binding of the secondary antibody to the anti-CD3 and anti-CD28 antibodies used for stimulating the cells.
Figure 2. Data for APC-Conjugated
Flow cytometry analysis of CD3+ cells (T cells) isolated from human peripheral blood mononuclear cells (PBMCs), then stimulated by incubation with anti-CD3 and anti-CD28 antibodies. Cells were labeled with Anti-Human CD71 (Transferrin Receptor) Antibody, Clone OKT9, APC (filled histogram) or a mouse IgG1, kappa isotype control antibody, APC (solid line histogram).
Figure 3. Data for Biotin-Conjugated
Flow cytometry analysis of CD3+ cells (T cells) isolated from human peripheral blood mononuclear cells (PBMCs), then stimulated by incubation with anti-CD3 and anti-CD28 antibodies. Cells were labeled with Anti-Human CD71 (Transferrin Receptor) Antibody, Clone OKT9, Biotin followed by streptavidin (SAV) APC (filled histogram), or a biotinylated mouse IgG1, kappa isotype control antibody followed by SAV APC (solid line histogram).
Figure 4. Data for FITC-Conjugated
Flow cytometry analysis of CD3+ cells (T cells) isolated from human peripheral blood mononuclear cells (PBMCs), then stimulated by incubation with anti-CD3 and anti-CD28 antibodies. Cells were labeled with Anti-Human CD71 (Transferrin Receptor) Antibody, Clone OKT9, FITC (filled histogram) or a mouse IgG1, kappa isotype control antibody, FITC (solid line histogram).
Figure 5. Data for PE-Conjugated
Flow cytometry analysis of CD3+ cells (T cells) isolated from human peripheral blood mononuclear cells (PBMCs), then stimulated by incubation with anti-CD3 and anti-CD28 antibodies. Cells were labeled with Anti-Human CD71 (Transferrin Receptor) Antibody, Clone OKT9, PE (filled histogram) or a mouse IgG1, kappa isotype control antibody, PE (solid line histogram).
This product is designed for use in the following research area(s) as part
of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we
offer to support each research area.
