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Anti-Human CD5 Antibody, Clone UCHT2

Mouse monoclonal IgG1 antibody against human, rhesus, cynomolgus CD5

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Anti-Human CD5 Antibody, Clone UCHT2

Mouse monoclonal IgG1 antibody against human, rhesus, cynomolgus CD5

From: 140 USD
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Mouse monoclonal IgG1 antibody against human, rhesus, cynomolgus CD5
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Overview

The UCHT2 antibody reacts with CD5, an ~67 kDa single-chain type I glycoprotein and member of the scavenger receptor superfamily, which is constitutively expressed on thymocytes, T cells, B cell subsets, peripheral blood dendritic cells and some leukemia and lymphoma cells, including chronic B lymphocytic leukemia (B-CLL) cells. CD5 is expressed at low levels on thymocytes and at high density on mature T cells. Putative ligands include CD5L and CD72. CD5 modulates T and B cell receptor signaling, thymocyte maturation, and T cell/B cell interactions via its physical and functional associations with the T cell receptor/CD3 complex and the B cell receptor. Studies with CD5 knockout mice indicate that CD5 negatively regulates antigen receptor-mediated signaling in thymocytes and mature T cells. The UCHT2 antibody binds to epitope 2 located in the extracellular domain of CD5.
Subtype
Primary Antibodies
Target Antigen
CD5
Alternative Names
Leu1, Leu-1, Ly-1, Lymphocyte antigen T1, T1, Tp67
Reactive Species
Capuchin Monkey, Chimpanzee, Common Marmoset, Cynomolgus, Human, Owl Monkey, Rhesus
Conjugation
APC, Biotin, FITC, PE, PerCP-Cyanine5.5, Unconjugated
Host Species
Mouse
Cell Type
T Cells
Species
Human, Non-Human Primate
Application
Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Western Blotting
Area of Interest
Immunology, Stem Cell Biology
Clone
UCHT2
Gene ID
921
Isotype
IgG1, kappa

Data Figures

Data for APC-Conjugated

Figure 1. Data for APC-Conjugated

(A) Flow cytometry analysis of human buffy coat nucleated cells labeled with Anti-Human CD5 Antibody, Clone UCHT2, APC and Anti-Human CD20 Antibody, Clone 2H7, Alexa Fluor® 488 (Catalog #60008AD). (B) Flow cytometry analysis of human buffy coat nucleated cells labeled with a mouse IgG1, kappa APC isotype control antibody and Anti-Human CD20 Antibody, Clone 2H7, Alexa Fluor® 488. (C) Flow cytometry analysis of human buffy coat nucleated cells processed with the EasySep™ HLA Whole Blood CD3 Positive Selection Kit and labeled with Anti-Human CD5 Antibody, Clone UCHT2, APC. Histograms show labeling of buffy coat nucleated cells (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa APC isotype control antibody is shown (solid line histogram).

Data for Biotin-Conjugated

Figure 2. Data for Biotin-Conjugated

(A) Flow cytometry analysis of human buffy coat nucleated cells labeled with Anti-Human CD5 Antibody, Clone UCHT2, Biotin followed by streptavidin (SAV) APC and Anti-Human CD20 Antibody, Clone 2H7, PE (Catalog #60008PE).
(B) Flow cytometry analysis of human buffy coat nucleated cells labeled with a mouse IgG1, kappa biotin isotype control antibody followed by streptavidin (SAV) APC and Anti-Human CD20 Antibody, Clone 2H7, PE.
(C) Flow cytometry analysis of human buffy coat nucleated cells processed with the EasySep™ HLA CD3 Positive Selection Kit and labeled with Anti-Human CD5 Antibody, Clone UCHT2, Biotin followed by streptavidin (SAV) APC. Histograms show labeling of buffy coat nucleated cells (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa biotin isotype control antibody followed by SAV APC is shown (open histogram).

Data for FITC-Conjugated

Figure 3. Data for FITC-Conjugated

(A) Flow cytometry analysis of human buffy coat nucleated cells labeled with Anti-Human CD5 Antibody, Clone UCHT2, FITC and Anti-Human CD20 Antibody, Clone 2H7, APC (Catalog #60008AZ). (B) Flow cytometry analysis of human buffy coat nucleated cells labeled with a mouse IgG1, kappa FITC isotype control antibody and Anti-Human CD20 Antibody, Clone 2H7, APC. (C) Flow cytometry analysis of human buffy coat nucleated cells processed with the EasySep™ HLA CD3 Positive Selection Kit and labeled with Anti-Human CD5 Antibody, Clone UCHT2, FITC. Histograms show labeling of buffy coat nucleated cells (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa FITC isotype control antibody is shown (solid line histogram).

Data for PE-Conjugated

Figure 4. Data for PE-Conjugated

(A) Flow cytometry analysis of human buffy coat nucleated cells labeled with Anti-Human CD5 Antibody, Clone UCHT2, PE and Anti-Human CD20 Antibody, Clone 2H7, APC (Catalog #60008AZ). (B) Flow cytometry analysis of human buffy coat nucleated cells labeled with a mouse IgG1, kappa PE isotype control antibody and Anti-Human CD20 Antibody, Clone 2H7, APC. (C) Flow cytometry analysis of human buffy coat nucleated cells processed with the EasySep™ HLA CD3 Positive Selection Kit and labeled with Anti-Human CD5 Antibody, Clone UCHT2, PE. Histograms show labeling of buffy coat nucleated cells (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa PE isotype control antibody is shown (solid line histogram).

Data for Unconjugated

Figure 5. Data for Unconjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD5 Antibody, Clone UCHT2, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (Catalog #60138FI) (filled histogram), or a mouse IgG1, kappa isotype control antibody, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (solid line histogram).

Data for PerCP-Cy55-Conjugated

Figure 6. Data for PerCP-Cy55-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD5 Antibody, Clone UCHT2, PerCP-Cy5.5 (filled histogram) or a mouse IgG1, kappa isotype control antibody, PerCP-Cy5.5 (solid line histogram).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
Anti-Human CD5 Antibody, Clone UCHT2, PE
Catalog #
60082PE.1, 60082PE
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD5 Antibody, Clone UCHT2, PerCP-Cy5.5
Catalog #
60082PS.1, 60082PS
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD5 Antibody, Clone UCHT2, Biotin
Catalog #
60082BT
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD5 Antibody, Clone UCHT2
Catalog #
60082
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD5 Antibody, Clone UCHT2, FITC
Catalog #
60082FI, 60082FI.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD5 Antibody, Clone UCHT2, APC
Catalog #
60082AZ, 60082AZ.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD5 Antibody, Clone UCHT2, PE
Catalog #
60082PE.1, 60082PE
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD5 Antibody, Clone UCHT2, PerCP-Cy5.5
Catalog #
60082PS.1, 60082PS
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD5 Antibody, Clone UCHT2, Biotin
Catalog #
60082BT
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD5 Antibody, Clone UCHT2
Catalog #
60082
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD5 Antibody, Clone UCHT2, FITC
Catalog #
60082FI, 60082FI.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD5 Antibody, Clone UCHT2, APC
Catalog #
60082AZ, 60082AZ.1
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (2)

Tools for Your Immunology Research
Brochure
Tools for Your Immunology Research
Human Immune Cytokines
Wallchart
Human Immune Cytokines

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Quality Statement:

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.

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