RoboSep™-C Human CD8+ T Cell Isolation Kit

Immunomagnetic negative selection kit
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Overview

The RoboSep™-C Human CD8+ T Cell Isolation Kit is designed to isolate untouched and highly purified CD8+ T cells from fresh human peripheral blood leukopaks using RoboSep™-C (Catalog #100-0185 and #100-0186) for automated immunomagnetic negative selection.

This kit targets non-CD8+ T cells for removal with antibodies recognizing specific cell surface markers. Using RoboSep™-C in a fully automated protocol, unwanted cells are labeled with antibodies and magnetic particles, and separated without columns using a RoboSep™-C magnet. The desired untouched cells are washed and concentrated by RoboSep™-C and transferred to a final product bag. Isolated cells are immediately available for downstream applications such as flow cytometry, culture, or cryopreservation.
Advantages
⦁ Fully automated large-scale isolation in a closed system
⦁ Up to 94% purity with high recovery
⦁ Untouched, viable cells
Components
  • RoboSep™-C Human CD8+ T Cell Isolation Cocktail, 10 mL
  • RoboSep™-C Dextran RapidSpheres™ 30106, 10 mL
Magnet Compatibility
• RoboSep™-C (Catalog #400-2274)
Subtype
Cell Isolation Kits
Cell Type
T Cells, T Cells, CD8+
Species
Human
Sample Source
Leukapheresis
Selection Method
Negative
Application
Cell Isolation
Brand
RoboSep
Area of Interest
Cell Therapy, Drug Discovery and Toxicity Testing, Immunology

Scientific Resources

Educational Materials (7)

Brochure
T Cell Reagents for Your Cellular Therapy Research
Brochure
Tools For Your Immunology Research
Brochure
RoboSep™-C: Automated Closed-System Cell Isolation
Technical Bulletin
Genome Editing of Human Primary T Cells Using CRISPR-Cas9
Wallchart
Production of Chimeric Antigen Receptor T Cells
Video
CAR T Cell Manufacturing Workflow: Isolation, Activation and Expansion
0:59
CAR T Cell Manufacturing Workflow: Isolation, Activation and Expansion
Webinar
RoboSep™-C - Automated Closed System for Large-Scale Human Immune Cell Isolation

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

FACS Profile Results with RoboSep™-C Human CD8+ T Cell Isolation Kit

Figure 1. Typical RoboSep™-C Human CD8+ T Cell Isolation Profile

Starting with a fresh human peripheral blood leukopak, the CD8+ T cell content (CD3+CD8+) of the isolated fraction is typically 86.8 ± 5.4% (mean ± SD). In the above example, the purities of the start and final isolated fractions are 16.5% and 93.3%, respectively.

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