ThawSTAR® CB Automated Thawing System

Automated high-volume cell thawing system for consistent performance

ThawSTAR® CB Automated Thawing System

Automated high-volume cell thawing system for consistent performance

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Automated high-volume cell thawing system for consistent performance
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Product Advantages


  • Reliably and consistently thaw samples with an automated thawing procedure driven by algorithm and biosample sensing technology

  • Eliminate risk of biosample loss and contamination with single-use barrier bags

  • Enjoy convenient set up and an intuitive user experience with the simple software interface

  • Use with common cryobag form factors from popular manufacturers

  • Prevent sample tracking errors with chain-of-custody solutions

Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.

Overview

Ensure consistent thawing of large-volume cryobags with ThawSTAR® CB. With a standardized thawing process that replaces manual, water bath-based thawing, ThawSTAR® CB reduces the risk of human error and standardizes cell thawing for scalable and reproducible results. Simply remove any external packaging and insert a frozen cryobag into a ThawSTAR® CB Barrier Bag to ensure proper positioning during thawing. Then select a Thaw Profile and retrieve the cryobag once the device alerts you at the end of the thaw cycle, after approximately 2 - 8 minutes. (See the ThawSTAR® CB User Manual for complete instructions). ThawSTAR® CB can be leveraged early in the R&D phase and scaled into commercial manufacturing.

ThawSTAR® CB is compatible with most major cryobag manufacturers and sizes, including STEMCELL’s Frozen Human Peripheral Blood Leukopaks (Catalog #200-0130/200-0131/200-0132).
Application
Cryopreservation
Brand
ThawSTAR
Area of Interest
Antibody Development, Immunology, Infectious Diseases

Data Figures

Figure 1. Frozen Human Peripheral Blood Leukopaks Thawed Using the ThawSTAR® CB Automated Thawing System Show High Recovery and Viability for Pre-wash Assessment

Frozen Human Peripheral Blood Leukopaks (Quarter Size; Catalog #200-0132) were retrieved from liquid nitrogen and thawed using either ThawSTAR® CB Automated Thawing System with appropriately selected thawing settings or a water bath. When thawed using the ThawSTAR® CB Automated Thawing System or a water bath, (A) the mean live cell recovery was 4.42 x 10⁹ vs. 3.58 x 10⁹ cells for Donor 1, 4.53 x 10⁹ vs. 4.95 x 10⁹ cells for Donor 2, 3.94 x 10⁹ vs. 4.52 x 10⁹ cells for Donor 3, respectively. (B) The mean viability of automatic vs. water bath-thawed cells was 93.3% vs. 95.4% for Donor 1, 98.9% vs. 98.3% for Donor 2, and 98.2% vs. 98.5% for Donor 3, respectively. The cells were from 3 different donors and were tested in triplicates. Cell recovery and viability was assessed using a NucleoView™ counter.

Figure 2. Frozen CD4+ Cells Thawed Using the ThawSTAR® CB Automated Thawing System Show High Recovery and Viability for Pre-wash Assessment

CD4+ cells cryopreserved in CryoStor® CS10 (Catalog #07930) were retrieved from liquid nitrogen and thawed using either ThawSTAR® CB Automated Thawing System with appropriately selected thawing settings or a water bath. When thawed using the ThawSTAR® CB Automated Thawing System or a water bath, (A) the mean live cell recovery was 1.27 x 10⁹ vs. 1.19 x 10⁹ cells for Donor 1, 1.03 x 10⁹ vs. 1.16 x 10⁹ cells for Donor 2, respectively and (B) the mean viability was 92.0% vs. 93.3% for Donor 1, 95.2% vs. 97.4% for Donor 2, respectively. The cells were from 2 different donors and were tested in triplicates. Cell recovery and viability were assessed using a NucleoView™ counter.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
100-1151
Lot #
All
Language
English
Document Type
Technical Manual
Catalog #
100-1151, 100-1151
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.