Anti-Human CD2 Antibody, Clone RPA-2.10

Mouse monoclonal IgG1 antibody against human, rhesus, cynomolgus CD2

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Mouse monoclonal IgG1 antibody against human, rhesus, cynomolgus CD2
From: 85 USD

Overview Related Products Scientific Resources Product Applications Data and Publications

Overview

The RPA-2.10 antibody reacts with CD2, an ~50 kDa type I transmembrane glycoprotein and a member of the immunoglobulin (Ig) superfamily; two Ig-like domains are located in its extracellular portion. CD2 is broadly expressed on peripheral T and NK cells, dendritic cells, erythrocytes, most thymic T cells, subsets of thymic B cells, and on the endothelium. CD2 expression appears early during T cell differentiation. Aberrant expression has been observed in some lymphomas and myeloid leukemias. CD2 is critically important for T cell activation and signaling, as well as lymphocyte adhesion. The primary ligand for CD2 is CD58 (LFA-3) located on antigen-presenting cells, with additional ligands comprising CD15 (SSEA-1), CD48 and CD59. Notably, the RPA-2.10 antibody blocks the mixed lymphocyte reaction.
Subtype:
Primary Antibodies
Target Antigen:
CD2
Alternative Names:
LFA-2, SRBC-R, T11
Reactive Species:
Baboon; Capuchin Monkey; Cynomolgus; Pigtailed Macaque; Rhesus; Squirrel Monkey; Chimpanzee; Human; Pig
Conjugation:
APC; FITC; PE; PerCP
Host Species:
Mouse
Cell Type:
T Cells
Application:
Cell Isolation; Flow Cytometry; Functional Assay; Immunocytochemistry; Immunofluorescence; Immunohistochemistry; Western Blotting
Area of Interest:
Immunology
Clone:
RPA-2.10
Gene ID:
914
Isotype:
IgG1, kappa

Scientific Resources

Product Documentation

Educational Materials

(5)
Brochure
Complete Antibody Listing
Brochure
HLA Antibodies for Purity Assessment
Brochure
Tools For Your Immunology Research
Brochure
Immunology Antibodies
Wallchart
Human Immune Cytokines

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area Workflow Stages for
Workflow Stages
Total Lymphocyte Research
Cell Sourcing
Cell Isolation
Cell Characterization
T Cell Research
Cell Sourcing
Cell Isolation
Cell Activation and Expansion
Cell Characterization
HLA Analysis
Cell Isolation
Characterization
T Cell Engineering
Cell Isolation
Cell Activation and Expansion
Cell Characterization
Cell Cryopreservation
Chimerism Analysis
Cell Isolation
Characterization

Data and Publications

Data

Data for APC-Conjugated

Figure 1. Data for APC-Conjugated

(A) Flow cytometry analysis of human buffy coat cells labeled with Anti-Human CD2 Antibody, Clone RPA-2.10, APC (filled histogram) or a mouse IgG1, kappa APC isotype control antibody (black line histogram).
(B) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) processed with the EasySep™ HLA CD3 Positive Selection Kit and labeled with Anti-Human CD2 Antibody, Clone RPA-2.10, APC. Histograms show labeling of PBMCs (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa APC isotype control antibody is shown (open histogram).

Data for Biotin-Conjugated

Figure 2. Data for Biotin-Conjugated

(A) Flow cytometry analysis of human buffy coat cells labeled with Anti-Human CD2 Antibody, Clone RPA-2.10, Biotin followed by streptavidin (SAV) APC (filled histogram) or a mouse IgG1, kappa biotin isotype control antibody followed by SAV APC (black line histogram).
(B) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) processed with the EasySep™ HLA CD3 Positive Selection Kit and labeled with Anti-Human CD2 Antibody, Clone RPA-2.10, Biotin followed by streptavidin (SAV) APC. Histograms show labeling of PBMCs (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa biotin isotype control antibody followed by SAV APC is shown (open histogram).

Data for FITC-Conjugated

Figure 3. Data for FITC-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD2 Antibody, Clone RPA-2.10, FITC and anti-human CD45 APC.
(B) Flow cytometry analysis of human PBMCs processed with the EasySep™ HLA CD3 Positive Selection Kit and labeled with Anti-Human CD2 Antibody, Clone RPA-2.10, FITC. Histograms show labeling of PBMCs (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa FITC isotype control antibody is shown (open histogram).

Data for PE-Conjugated

Figure 4. Data for PE-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD2 Antibody, Clone RPA-2.10, PE and anti-human CD45 APC. (B) Flow cytometry analysis of human PBMCs processed with the EasySep™ HLA CD3 Positive Selection Kit and labeled with Anti-Human CD2 Antibody, Clone RPA-2.10, PE. Histograms show labeling of PBMCs (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa PE isotype control antibody is shown (open histogram).

Data for Unconjugated

Figure 5. Data for Unconjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD2 Antibody, Clone RPA-2.10, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (Catalog #60138FI; filled histogram), or a mouse IgG1, kappa isotype control antibody followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (solid line histogram).

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.