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GloCell™ Fixable Viability Dye Violet 450

Amine-labeling fluorescent dye for live/dead staining of mammalian cells

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From: 85 USD

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Amine-labeling fluorescent dye for live/dead staining of mammalian cells
From: 85 USD

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Overview

GloCell™ Fixable Viability Dye Violet 450 is a fluorescent cell viability dye for staining live/dead mammalian cells in applications such as flow cytometry. The dye irreversibly binds intracellular and cell surface amine groups and can be utilized prior to fixation, permeabilization, or cryopreservation. Cells with compromised plasma membranes become permeable to the GloCell™ dye, resulting in a greater fluorescence compared to live cells in a cell viability assay.


GloCell™ Fixable Viability Dye Violet 450 is excited by the violet laser at 405 nm and it has a fluorescence emission maximum of 450 nm that can be detected using the 440/40 or 440/50 band pass filter used to detect Pacific Blue™.
Application:
Flow Cytometry; Immunofluorescence
Brand:
GloCell
Area of Interest:
Angiogenic Cell Research; Antibody Development; Cancer Research; Cell Line Development; Chimerism; Disease Modeling; Drug Discovery and Toxicity Testing; Endothelial Cell Biology; Epithelial Cell Biology; HIV; HLA; Hybridoma Generation; Immunology; Infectious Diseases; Metabolism; Mitosis; Neuroscience; Organoids; Respiratory Research; Stem Cell Biology; T Cell Engineering; Transplantation Research

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Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

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Data and Publications

Data

Figure 1.

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with GloCell™ Fixable Viability Dye Violet 450. (B) Flow cytometry analysis of human multiple myeloma bone marrow mononuclear cells (BMMCs) labeled with GloCell™ Fixable Viability Dye Violet 450.

Figure 2.

Excitation/Emission spectra for GloCell™ Fixable Viability Dye Violet 450. Excitation max: 403 nm, Emission Max: 454 nm

STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.