GloCell™ Fixable Viability Dye Violet 450

Amine-labeling fluorescent dye for live/dead staining of mammalian cells

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Amine-labeling fluorescent dye for live/dead staining of mammalian cells
From: 85 USD

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Overview Related Products Scientific Resources Product Applications Data and Publications

Overview

GloCell™ Fixable Viability Dye Violet 450 is a fluorescent cell viability dye for staining live/dead mammalian cells in applications such as flow cytometry. The dye irreversibly binds intracellular and cell surface amine groups and can be utilized prior to fixation, permeabilization, or cryopreservation. Cells with compromised plasma membranes become permeable to the GloCell™ dye, resulting in a greater fluorescence compared to live cells in a cell viability assay.


GloCell™ Fixable Viability Dye Violet 450 is excited by the violet laser at 405 nm and it has a fluorescence emission maximum of 450 nm that can be detected using the 440/40 or 440/50 band pass filter used to detect Pacific Blue™.
Application:
Flow Cytometry; Immunofluorescence
Brand:
GloCell
Area of Interest:
Angiogenic Cell Research; Antibody Development; Cancer Research; Cell Line Development; Chimerism; Disease Modeling; Drug Discovery and Toxicity Testing; Endothelial Cell Biology; Epithelial Cell Biology; HIV; HLA; Hybridoma Generation; Immunology; Infectious Diseases; Metabolism; Mitosis; Neuroscience; Organoids; Respiratory Research; Stem Cell Biology; T Cell Engineering; Transplantation Research

Scientific Resources

Product Documentation

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area Workflow Stages for
Workflow Stages
B Cell Research
Cell Sourcing
Cell Isolation
Cell Characterization
Brain Tumor Stem Cell Research
Tissue Dissociation
Expansion
Differentiation
Characterization
Airway Epithelial Cell Research
Expansion
Differentiation
CHO and Other Cell Line Development
Transfection and Selection
Cloning
Expansion and Characterization
Dendritic Cell Research
Cell Sourcing
Cell Isolation
Differentiation and Maturation
Cell Characterization
Human Pluripotent Stem Cell Research
Reprogramming
Maintenance
Differentiation
Granulocyte Research
Cell Sourcing
Cell Isolation
Cell Characterization
Hematopoietic Stem and Progenitor Cell Research
Cell Sourcing and Isolation
Expansion and Differentiation
Analysis
hPSC-Derived Endoderm Cell Research
Endoderm Induction
Characterization and Isolation
Downstream Differentiation
hPSC-Derived Neural Cell Research
Cell Sourcing
Neural Induction
Expansion
Differentiation and Maturation
Characterization
hPSC-Derived Mesoderm Cell Research
Mesoderm Induction
Downstream Differentiation
Hybridoma Production
Pre-Enrichment and Fusion
Selection and Cloning
Expansion and Characterization
Intestinal Epithelial Cell Research
Tissue Dissociation
Cell Culture
Total Lymphocyte Research
Cell Sourcing
Cell Isolation
Cell Characterization
Mammary Epithelial Cell Research
Tissue Dissociation and Isolation
Cell Culture and Assay
Characterization
Mesenchymal Stem and Progenitor Cell Research
Cell Sourcing and Isolation
Culture and Cryopreservation
Differentiation
Characterization
Monocyte Research
Cell Sourcing
Cell Isolation
Differentiation and Maturation
Cell Characterization
Mouse Pluripotent Stem Cell Research
Reprogramming
Maintenance
Differentiation
Myeloid Cell Research
Cell Sourcing
Cell Isolation
Cell Characterization
Neural Stem and Progenitor Cell Research
Tissue Dissociation
Expansion
Differentiation
Characterization
Neuronal Research
Tissue Dissociation
Cell Culture
Characterization
Natural Killer Cell Research
Cell Sourcing
Cell Isolation
Cell Characterization
Prostate Epithelial Cell Research
Tissue Dissociation and Isolation
Cell Culture and Assay
Characterization
T Cell Research
Cell Sourcing
Cell Isolation
Cell Activation and Expansion
Cell Characterization
HLA Analysis
Cell Isolation
Characterization
Innate Lymphoid Cell Research
Cell Sourcing
Cell Isolation
Cell Culture and Characterization

Data and Publications

Data

Figure 1.

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with GloCell™ Fixable Viability Dye Violet 450. (B) Flow cytometry analysis of human multiple myeloma bone marrow mononuclear cells (BMMCs) labeled with GloCell™ Fixable Viability Dye Violet 450.

Figure 2.

Excitation/Emission spectra for GloCell™ Fixable Viability Dye Violet 450. Excitation max: 403 nm, Emission Max: 454 nm

STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.