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This rabbit monoclonal antibody (clone 2E1) reacts with human, mouse and rat sex determining region Y-box 2 (SOX2), part of the SOX transcription factor family. Use this recombinant monoclonal antibody in applications requiring detection of SOX2, such as ELISA, western blot (WB), immunohistochemistry (IHC), immunocytochemistry (ICC), and immunofluorescence (IF). This antibody clone has been verified for labeling SOX2-positive hPSC-derived neural stem and progenitor cells generated with STEMdiff™ SMADi Neural Induction Kit (Catalog #08581) and STEMdiff™ Neural Progenitor Medium (Catalog #05833) as well as human pluripotent stem cells grown with mTeSR™ Plus (Catalog #100-0276).
SOX2 plays a key role in maintaining the pluripotency of embryonic stem (ES) cells; if SOX2 levels are not regulated, high or low expression of SOX2 will result in the loss of ES cell pluripotency. In addition, SOX2 plays a key role in the development of pluripotent cells by embryos, and as such is integral in embryonic development. SOX transcription factors possess a common high-mobility group (HMG) DNA binding protein that is common between all members of the family. SOX2 in particular is classified as part of the SOXB1 group that also contains members SOX1 and SOX3. SOX transcription factors propagate their functions in cell differentiation and development via protein nuclear localization. This mechanism utilizes nuclear localization signals (NLS) that are situated on either extremity of the DNA-binding HMG-box domain. When these regions are afflicted by mutations, this may result in cancers as well as a variety of developmental diseases.
Figure 1. Human Neural Progenitor Cells Stained with Anti-Human SOX2 Antibody, Polyclonal
(A) Human neural progenitor cells (NPCs) were generated from induced pluripotent stem (iPS) cells using STEMdiff™ SMADi Neural Induction Kit and STEMdiff™ Neural Progenitor Medium and cultured on Corning® Matrigel®. NPCs were then fixed and labeled with Anti-Human SOX2 Antibody, followed by Goat Anti-Rabbit IgG (H+L) Antibody, Polyclonal, iFluor™ 488 (green) (Catalog #100-1082). Inset shows NPCs labeled with a rabbit IgG isotype control antibody, followed by Goat Anti-Rabbit IgG (H+L) Antibody, Polyclonal, iFluor™ 488 (with DAPI staining). (B) DAPI (blue) counterstaining of the cells shown in figure (A); nuclear localization of SOX2 is evident.
Figure 2. Human iPS Cells Stained with Anti-Human SOX2 Antibody, Polyclonal
(A) Human iPS cells were cultured in mTeSR™ Plus on Corning® Matrigel®, then fixed and labeled with Anti-Human SOX2 Antibody, followed by Goat Anti-Rabbit IgG (H+L) Antibody, Polyclonal, iFluor™ 488 (green) (Catalog #100-1082). Inset shows iPS cells labeled with a rabbit IgG isotype control antibody, followed by Goat Anti-Rabbit IgG (H+L) Antibody, Polyclonal, iFluor™ 488 (with DAPI staining). (B) DAPI (blue) counterstaining of the cells shown in figure (A); nuclear localization of SOX2 is evident.
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cGMP, stabilized feeder-free maintenance medium for human ES and iPS cells
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Anti-Human SOX2 (ANOP3) Antibody, Clone 2E1
Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.