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Data

Figure 1. Data for Unconjugated
(A) E18 cortical rat neurons were cultured using NeuroCult™ SM1 Neuronal Culture Kit on poly-lysine-coated glass coverslips, then fixed and labeled with Anti-Human Beta-Tubulin III Antibody, Clone 2G10-TB3, followed by donkey anti-mouse IgG, Alexa Fluor® 488. Nuclei were counterstained with DAPI. Inset shows cells labeled with a mouse IgG2a, kappa isotype control antibody followed by donkey anti-mouse IgG, Alexa Fluor® 488, and counterstained with DAPI. (B) Western blot analysis of denatured/reduced cell lysates with Anti-Beta Tubulin III Antibody, Clone 2G10-TB3. Lane 1, adult rat brain cortical cells; lane 2 (negative control), mouse E13.5 neural progenitor cells cultured with NeuroCult™ Proliferation Kit (Mouse & Rat).