ES-Cult™ M3120

Base methylcellulose medium for in vitro differentiation of mouse ES or iPS cells to hematopoietic or endothelial progenitor cells

ES-Cult™ M3120

Base methylcellulose medium for in vitro differentiation of mouse ES or iPS cells to hematopoietic or endothelial progenitor cells

ES-Cult™ M3120
40 mL
116 USD
Catalog # 03120

Base methylcellulose medium for in vitro differentiation of mouse ES or iPS cells to hematopoietic or endothelial progenitor cells

Overview

ES-Cult™ M3120 is recommended as a base for the preparation of methylcellulose-based medium for the in vitro differentiation of mouse embryonic stem (ES) or induced pluripotent stem (iPS) cells into hematopoietic or endothelial progenitor cells. When supplemented with the appropriate additional components, ES-Cult™ M3120 can be used for the generation of embryoid bodies (EBs) from undifferentiated mouse ES or iPS cells.
Contains
• 2.6% Methylcellulose
• Iscove’s MDM
Subtype
Semi-Solid Media, Specialized Media
Cell Type
Pluripotent Stem Cells
Species
Mouse
Application
Cell Culture, Differentiation
Brand
ES-Cult
Area of Interest
Stem Cell Biology
Formulation
Serum-Free

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Product Name
ES-Cult™ M3120
Catalog #
03120
Lot #
All
Language
English
Document Type
Technical Manual
Product Name
ES-Cult™ M3120
Catalog #
03120
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
ES-Cult™ M3120
Catalog #
03120
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Resources and Publications

Publications (1)

Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells Yu J et al. Yearbook of Dermatology and Dermatologic Surgery 2008 DEC

Abstract

Somatic cell nuclear transfer allows trans-acting factors present in the mammalian oocyte to reprogram somatic cell nuclei to an undifferentiated state. We show that four factors (OCT4, SOX2, NANOG, and LIN28) are sufficient to reprogram human somatic cells to pluripotent stem cells that exhibit the essential characteristics of embryonic stem (ES) cells. These induced pluripotent human stem cells have normal karyotypes, express telomerase activity, express cell surface markers and genes that characterize human ES cells, and maintain the developmental potential to differentiate into advanced derivatives of all three primary germ layers. Such induced pluripotent human cell lines should be useful in the production of new disease models and in drug development, as well as for applications in transplantation medicine, once technical limitations (for example, mutation through viral integration) are eliminated.

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