MesenCult™-ACF Plus Umbilical Cord Culture Kit

Animal component-free medium for derivation and expansion of human mesenchymal stromal cells from umbilical cord tissue
MesenCult™-ACF Plus Umbilical Cord Culture Kit

Animal component-free medium and substrate for derivation and expansion of human mesenchymal stromal cells from umbilical cord tissue

1 Kit
Catalog # 100-0234
620 USD
Required Products
  1. Animal Component-Free Cell Dissociation Kit
    Animal Component-Free Cell Dissociation Kit

    Dissociation kit for human stem and progenitor cells

  2. L-Glutamine
    L-Glutamine

    Cell culture supplement

  3. D-PBS Without Ca++ and Mg++
    D-PBS (Without Ca++ and Mg++)

    Dulbecco’s phosphate-buffered saline without calcium and magnesium

Overview

MesenCult™-ACF Plus Umbilical Cord Culture Kit includes standardized, animal component- and serum-free media for the derivation and expansion of human mesenchymal stromal cells (also known as mesenchymal stem cells; MSCs) from umbilical cord tissue. This kit
works together with complete MesenCult™-ACF Plus Medium for the long-term expansion of umbilical cord-derived MSCs.

MesenCult™-ACF Plus Umbilical Cord Medium must be used in conjunction with Animal Component-Free Cell Attachment Substrate and Animal Component-Free Cell Dissociation Kit (Catalog #05426) to provide a complete animal component-free (ACF) culture system. Components of Animal Component-Free Cell Attachment Substrate and Animal Component-Free Cell Dissociation Kit are pre-screened and tested for optimal cell adherence when cells are cultured with MesenCult™-ACF Plus Umbilical Cord Culture Kit and MesenCult™-ACF Plus Medium. For optimal animal component-free cryopreservation, MesenCult™-ACF Freezing Medium (Catalog #05490) is recommended for human MSCs previously cultured in MesenCult™ media.

NOTE: L-Glutamine (Catalog #07100) is required for preparation of MesenCult™-ACF Plus Umbilical Cord Medium and complete MesenCult™-ACF Plus Medium; see Preparation of Reagents and Materials.
Advantages
⦁ Supports high yield of MSCs from umbilical cord tissue
⦁ Umbilical cord-derived MSCs consistently express MSC-specific markers and retain superior expansion and differentiation capabilities
⦁ Complete animal component-free workflow compatible with MesenCult™-ACF portfolio
Components
  • MesenCult™-ACF Plus Medium, 500 mL
  • MesenCult™ Umbilical Cord Derivation 500X Supplement, 0.5 mL
  • MesenCult™-ACF Plus 500X Supplement, 0.5 mL
  • Animal Component-Free Cell Attachment Substrate, 3 x 1 mL
Subtype
Specialized Media
Cell Type
Mesenchymal Stromal Cells
Species
Human
Application
Cell Culture, Cell Isolation
Brand
MesenCult
Area of Interest
Cell Line Development, Stem Cell Biology
Formulation
Animal Component-Free

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
MesenCult™-ACF Plus Umbilical Cord Culture Kit
Catalog #
100-0234
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
MesenCult™-ACF Plus Umbilical Cord Culture Kit
Catalog #
100-0234
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
MesenCult™-ACF Plus Umbilical Cord Culture Kit
Catalog #
100-0234
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
MesenCult™-ACF Plus Umbilical Cord Culture Kit
Catalog #
100-0234
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
MesenCult™-ACF Plus Umbilical Cord Culture Kit
Catalog #
100-0234
Lot #
All
Language
English

Educational Materials(8)

Brochure
Products for Your Mesenchymal Stromal Cell Research
Technical Bulletin
Extracellular Vesicle Generation from Mesenchymal Stromal Cells Using MesenCult™-ACF Plus
Wallchart
The Identity and Properties of Mesenchymal Stem Cells
Wallchart
Mesenchymal Stromal Cell-Derived Exosomes: Biogenesis and Cargoes
Video
13:45
Isolation of Umbilical Cord Mesenchymal Stromal Cells Using Explant Method
Video
5:34
How to Isolate Mesenchymal Stromal Cells (MSCs) from Mouse Compact Bone
Video
1:51
MesenCult™ for Mesenchymal Stromal Cell Derivation, Culture & Differentiation
Webinar
58:00
The Secret In Vivo Life of "Mesenchymal Stem Cells"

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Contact STEMCELL Technologies

Our Customer Service, Sales, and Product and Scientific Support departments in North America are available between 6 am and 5 pm Pacific Time (9 am and 8 pm Eastern Time). One of our representatives will be happy to help you by telephone or email. Please complete the form to contact us by email. A representative will get back to you shortly.
  •  

StemCell Technologies Inc. and affiliates ("STEMCELL Technologies") does not share your email address with third parties. StemCell Technologies Inc. will use your email address to confirm your identity and send you newsletters, transaction-related emails, promotional and customer service emails in accordance with our privacy policy. You can change your email preferences at any time.