MesenCult™-ACF Plus Umbilical Cord Culture Kit
Animal component-free medium for derivation and expansion of human mesenchymal stromal cells from umbilical cord tissue
Required Products
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Animal Component-Free Cell Dissociation KitDissociation kit for human stem and progenitor cells
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L-GlutamineCell culture supplement
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D-PBS (Without Ca++ and Mg++)Dulbecco’s phosphate-buffered saline without calcium and magnesium
Overview
MesenCult™-ACF Plus Umbilical Cord Culture Kit includes standardized, animal component- and serum-free media for the derivation and expansion of human mesenchymal stromal cells (also known as mesenchymal stem cells; MSCs) from umbilical cord tissue. This kit
works together with complete MesenCult™-ACF Plus Medium for the long-term expansion of umbilical cord-derived MSCs.
MesenCult™-ACF Plus Umbilical Cord Medium must be used in conjunction with Animal Component-Free Cell Attachment Substrate and Animal Component-Free Cell Dissociation Kit (Catalog #05426) to provide a complete animal component-free (ACF) culture system. Components of Animal Component-Free Cell Attachment Substrate and Animal Component-Free Cell Dissociation Kit are pre-screened and tested for optimal cell adherence when cells are cultured with MesenCult™-ACF Plus Umbilical Cord Culture Kit and MesenCult™-ACF Plus Medium. For optimal animal component-free cryopreservation, MesenCult™-ACF Freezing Medium (Catalog #05490) is recommended for human MSCs previously cultured in MesenCult™ media.
NOTE: L-Glutamine (Catalog #07100) is required for preparation of MesenCult™-ACF Plus Umbilical Cord Medium and complete MesenCult™-ACF Plus Medium; see Preparation of Reagents and Materials.
works together with complete MesenCult™-ACF Plus Medium for the long-term expansion of umbilical cord-derived MSCs.
MesenCult™-ACF Plus Umbilical Cord Medium must be used in conjunction with Animal Component-Free Cell Attachment Substrate and Animal Component-Free Cell Dissociation Kit (Catalog #05426) to provide a complete animal component-free (ACF) culture system. Components of Animal Component-Free Cell Attachment Substrate and Animal Component-Free Cell Dissociation Kit are pre-screened and tested for optimal cell adherence when cells are cultured with MesenCult™-ACF Plus Umbilical Cord Culture Kit and MesenCult™-ACF Plus Medium. For optimal animal component-free cryopreservation, MesenCult™-ACF Freezing Medium (Catalog #05490) is recommended for human MSCs previously cultured in MesenCult™ media.
NOTE: L-Glutamine (Catalog #07100) is required for preparation of MesenCult™-ACF Plus Umbilical Cord Medium and complete MesenCult™-ACF Plus Medium; see Preparation of Reagents and Materials.
Advantages
⦁ Supports high yield of MSCs from umbilical cord tissue
⦁ Umbilical cord-derived MSCs consistently express MSC-specific markers and retain superior expansion and differentiation capabilities
⦁ Complete animal component-free workflow compatible with MesenCult™-ACF portfolio
⦁ Umbilical cord-derived MSCs consistently express MSC-specific markers and retain superior expansion and differentiation capabilities
⦁ Complete animal component-free workflow compatible with MesenCult™-ACF portfolio
Components
- MesenCult™-ACF Plus Medium, 500 mL
- MesenCult™ Umbilical Cord Derivation 500X Supplement, 0.5 mL
- MesenCult™-ACF Plus 500X Supplement, 0.5 mL
- Animal Component-Free Cell Attachment Substrate, 3 x 1 mL
Subtype
Specialized Media
Cell Type
Mesenchymal Stromal Cells
Species
Human
Application
Cell Culture, Cell Isolation
Brand
MesenCult
Area of Interest
Cell Line Development, Stem Cell Biology
Formulation
Animal Component-Free
Related Products
Related Products
Scientific Resources
Product Documentation
| Document Type | Product Name | Catalog # | Lot # | Language |
|---|---|---|---|---|
|
Document Type
Product Information Sheet
|
Product Name
MesenCult™-ACF Plus Umbilical Cord Culture Kit
|
Catalog #
100-0234 |
Lot #
All |
Language
English |
|
Document Type
Safety Data Sheet 1
|
Product Name
MesenCult™-ACF Plus Umbilical Cord Culture Kit
|
Catalog #
100-0234 |
Lot #
All |
Language
English |
|
Document Type
Safety Data Sheet 2
|
Product Name
MesenCult™-ACF Plus Umbilical Cord Culture Kit
|
Catalog #
100-0234 |
Lot #
All |
Language
English |
|
Document Type
Safety Data Sheet 3
|
Product Name
MesenCult™-ACF Plus Umbilical Cord Culture Kit
|
Catalog #
100-0234 |
Lot #
All |
Language
English |
|
Document Type
Safety Data Sheet 4
|
Product Name
MesenCult™-ACF Plus Umbilical Cord Culture Kit
|
Catalog #
100-0234 |
Lot #
All |
Language
English |
Educational Materials (8)
Brochure
Products for Your Mesenchymal Stromal Cell Research
Technical Bulletin
Extracellular Vesicle Generation from Mesenchymal Stromal Cells Using MesenCult™-ACF Plus
Wallchart
The Identity and Properties of Mesenchymal Stem Cells
Wallchart
Mesenchymal Stromal Cell-Derived Exosomes: Biogenesis and Cargoes
Video
13:45
Isolation of Umbilical Cord Mesenchymal Stromal Cells Using Explant Method
Video
5:34
How to Isolate Mesenchymal Stromal Cells (MSCs) from Mouse Compact Bone
Video
1:51
MesenCult™ for Mesenchymal Stromal Cell Derivation, Culture & Differentiation
Webinar
58:00
The Secret In Vivo Life of "Mesenchymal Stem Cells"
Product Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Research Area
Workflow Stages
Legal Statement:
The included rhCollagen is supplied by CollPlant.
Quality Statement:
STEMCELL TECHNOLOGIES INC.'S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.
