Mesenchymal Stem and Progenitor Cell Research
MSCs, originally coined mesenchymal stem cells1 but now also referred to by other terms, such as multipotent mesenchymal stromal cells,2,3 mesenchymal progenitor cells4 or medicinal signaling cells,5 occur at low frequency in tissues and must be expanded in vitro to obtain sufficient numbers for basic and translational research.
Products
Tissue and Cell Culture Dissociation Reagents (4 1)
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Trypsin-EDTA (0.25%)
Enzymatic cell dissociation reagent
Cell Storage Media (4 3)
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CryoStor® CS5
Animal component-free, defined cryopreservation medium with 5% DMSO -
CryoStor® CS10
Animal component-free, defined cryopreservation medium with 10% DMSO -
CryoStor® CS2
Animal component-free, defined cryopreservation medium with 2% DMSO
Cell Dyes and Detection Assay Kits (4 8)
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3% Acetic Acid with Methylene Blue
Reagent for manual counting of nucleated mammalian cells -
GloCell™ Fixable Viability Dye Violet 540
Amine-labeling fluorescent dye for live/dead staining of mammalian cells -
GloCell™ Fixable Viability Dye Violet 510
Amine-labeling fluorescent dye for live/dead staining of mammalian cells -
GloCell™ Fixable Viability Dye Violet 450
Amine-labeling fluorescent dye for live/dead staining of mammalian cells
Buffers and Solutions (4 1)
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D-PBS (Without Ca++ and Mg++)
Dulbecco’s phosphate-buffered saline without calcium and magnesium
Small Molecules (4 6)
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Bafilomycin A1
Inhibits autophagy and vacuolar H+-ATPase (V-ATPase) -
Ascorbic Acid
Antioxidant; Reducing agent -
BIRB-796
p38 MAPK inhibitor -
(-)-Blebbistatin
Non-muscle myosin II (NM II) ATPase inhibitor
Laboratory Equipment (4 2)
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Hypoxia Incubator Chamber
Chamber for generation of a hypoxic environment for tissue culture -
Single Flow Meter
Meter to control gas flow into hypoxia incubator chamber
Cultureware and General Supplies (4 5)
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Costar® 24-Well Flat-Bottom Plate, Tissue Culture-Treated
Clear polystyrene flat-bottom, tissue culture-treated multiwell cell culture plate with lid -
Falcon® 6-Well Flat-Bottom Plate, Tissue Culture-Treated
Clear polystyrene flat-bottom, tissue culture-treated multiwell plate with lid -
Costar® 6-Well Flat-Bottom Plate, Tissue Culture-Treated
Clear polystyrene flat-bottom, tissue culture-treated multiwell plate with lid -
Tissue Culture-Treated Dishes, 100 mm
100 mm treated tissue culture dishes