Mesenchymal Stem and Progenitor Cell Research

MSCs, originally coined mesenchymal stem cells¹ but now also referred to by other terms, such as multipotent mesenchymal stromal cells,^2,3 mesenchymal progenitor cells⁴ or medicinal signaling cells,⁵ occur at low frequency in tissues and must be expanded in vitro to obtain sufficient numbers for basic and translational research. See More

Serum-containing (for human and mouse cells) and serum-free (for human cells) MesenCult™ media are optimized for efficient expansion of MSCs in culture and the quantification of functional MSCs in the colony-forming unit - fibroblast (CFU-F) assay. With standardized, xeno-free and animal component-free media formulations available, concerns about immune rejection of transplanted cells or disease transmission are addressed.⁶

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References

Caplan AI et al. (1991) J Orthop Res 9(5): 641-650
Horwitz EM et al. (2005) Cytotherapy 7(5) 393-5
Dominici M et al. (2006) Cytotherapy 8(4) 315-7
Shenaq DS. et al. (2010) Stem Cells Int doi: 10.4061/2010/519028
Caplan AI. (2010) Tissue Eng Part A 16(8) 2415-7
Thirumala S et al. (2013) Expert Opin Biol Ther 13(5): 673-91

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Products

Cell Culture Media and Supplements 4 (8)

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MesenCult™-ACF Plus Culture Kit

Animal component-free medium and attachment substrate for human mesenchymal stem cells
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MesenCult™-ACF Plus Medium

Animal component-free medium for human mesenchymal stem cells
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MesenCult™-hPL Medium Kit

For the culture of human mesenchymal stem cells
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L-Glutamine

Cell culture supplement

Tissue and Cell Culture Dissociation Reagents 4 (2)

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Trypsin-EDTA (0.25%)

Enzymatic cell dissociation reagent
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Animal Component-Free Cell Dissociation Kit

Dissociation kit for human stem and progenitor cells

Matrices and Substrates 4 (1)

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MesenCult™-SF Attachment Substrate

Serum-free attachment substrate for human mesenchymal stem cells

Cell Storage Media 4 (5)

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CryoStor® CS2

Animal component-free, defined cryopreservation medium with 2% DMSO
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MesenCult™-ACF Freezing Medium

Animal component-free MSC cryopreservation medium
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CryoStor® CS5

Animal component-free, defined cryopreservation medium with 5% DMSO
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CryoStor® CSB

Animal component-free and protein-free cryopreservation formulation

Cell Dyes and Detection Assay Kits 4 (8)

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3% Acetic Acid with Methylene Blue

Reagent for manual counting of nucleated mammalian cells
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Trypan Blue

Reagent for counting viable mammalian cells
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GloCell™ Fixable Viability Dye Violet 540

Amine-labeling fluorescent dye for live/dead staining of mammalian cells
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GloCell™ Fixable Viability Dye Violet 510

Amine-labeling fluorescent dye for live/dead staining of mammalian cells

Buffers and Solutions 4 (1)

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D-PBS (Without Ca++ and Mg++)

Dulbecco’s phosphate-buffered saline without calcium and magnesium

Cytokines and Proteins 4 (21)

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Human Recombinant TGF-beta 1 (Cell Culture Ready)

Ready-to-use transforming growth factor beta 1
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Human Recombinant PDGF-DD

Platelet-derived growth factor DD
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Human Recombinant FGF-7, ACF

Fibroblast growth factor 7, animal component-free
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Human Recombinant FGF-10 (KGF-2), ACF

Fibroblast growth factor 10, animal component-free

Small Molecules 4 (5)

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Bafilomycin A1

Inhibits autophagy and vacuolar H+-ATPase (V-ATPase)
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BIRB-796

p38 MAPK inhibitor
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(-)-Blebbistatin

Non-muscle myosin II (NM II) ATPase inhibitor
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CHIR99021

WNT pathway activator; Inhibits GSK3

Laboratory Equipment 4 (2)

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Single Flow Meter

Meter to control gas flow into hypoxia incubator chamber
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Hypoxia Incubator Chamber

Chamber for generation of a hypoxic environment for tissue culture

Cultureware and General Supplies 4 (3)

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Costar® 24-Well Flat-Bottom Plate, Tissue Culture-Treated

Clear polystyrene flat-bottom, tissue culture-treated multiwell cell culture plate with lid
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Falcon® 6-Well Flat-Bottom Plate, Tissue Culture-Treated

Clear polystyrene flat-bottom, tissue culture-treated multiwell plate with lid
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Costar® 6-Well Flat-Bottom Plate, Tissue Culture-Treated

Clear polystyrene flat-bottom, tissue culture-treated multiwell plate with lid

Primary and Cultured Cells 4 (3)

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Human Platelet Lysate, Fibrinogen-Depleted, Xeno-Free

Growth factor-rich supplement for the expansion of cells in vitro
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Human Platelet Lysate, Fibrinogen-Depleted, GMP-Compliant

Growth factor-rich supplement for the expansion of cells in vitro
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Human Platelet Lysate, Fibrinogen-Depleted

Growth factor-rich supplement for the expansion of cells in vitro

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