Deoxyribonuclease I (DNase I) is an endonuclease consisting of a single glycosylated polypeptide chain with two disulfide bonds. DNase is often included in tissue dissociation protocols to digest DNA that has leaked into the dissociation medium as a result of cell damage. DNase I preferentially cleaves phosphodiester linkages adjacent to pyrimidine nucleotides in both single- and double-stranded DNA, yielding polynucleotides with 5’-phosphate and 3’-hydroxyl groups (Bernardi et al.). DNase I has been used for the digestion of human tissues such as microglia (Klegeris & McGeer), cartilage (Dunham & Koch), colon (Fukushima & Fiocchi), epithelium (Fukushima & Fiocchi), liver (Vatakis et al.), lung (Fujino et al.), neural (Fuja et al.), and stem cells (Kusuma et al.).
DNA endonuclease; DNA nuclease; Deoxyribonucleic phosphatase; Pancreatic DNase; Thymonuclease
B Cells, Endothelial Colony Forming Cells (ECFCs), Neurons, Osteoblasts, T Cells
Reversible strainer for filtration of single cells and isolation of cellular aggregates, including embryoid bodies and spheroids
Human Immune Cytokines
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