Liver Epithelial Cell Research
Liver stem cells are dormant and generally not detectable in the healthy adult liver. However, injury triggers the activation of an Lgr5+ epithelial progenitor cell population. Single Lgr5+ cells from adult mouse liver can be isolated and expanded into a 3D culture system to generate hepatic organoids that possess clonogenic potential and can be maintained long-term. Use HepatiCult™ Organoid Growth Medium (Mouse) to establish and maintain hepatic organoids from mouse ductal fragments. For human cultures, Epcam+ bile duct-derived bipotent progenitors can be isolated for expansion into human hepatic organoids. Use HepatiCult™ Organoid Kit (Human) to generate mature hepatic organoids from fresh or frozen human liver tissue.
Products
Cell Culture Media and Supplements 4 (1)
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DMEM/F-12 with 15 mM HEPES
Dulbecco's Modified Eagle's Medium/Nutrient Ham's Mixture F-12 (DMEM/F-12) with 15 mM HEPES buffer
Cell Dyes and Detection Assay Kits 4 (2)
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DAPI (Hydrochloride)
DNA-labeling dye -
Trypan Blue
Reagent for counting viable mammalian cells
Buffers and Solutions 4 (1)
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D-PBS (Without Ca++ and Mg++)
Dulbecco’s phosphate-buffered saline without calcium and magnesium
Tissue and Cell Culture Dissociation Reagents 4 (3)
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Collagenase A, ACF
Animal component-free collagenase for the digestion of native collagen fibrils -
Collagenase Type I
For digestion of native collagen fibrils -
Gentle Cell Dissociation Reagent
cGMP, enzyme-free cell dissociation reagent
Cultureware and General Supplies 4 (1)
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Reversible Strainers
Reversible strainer for filtration of single cells and isolation of cellular aggregates, including embryoid bodies and spheroids
Primary and Cultured Cells 4 (1)
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Mouse Intestinal Organoids
Cryopreserved mouse intestinal epithelial organoids for establishment of organoid cultures
