Corning® Matrigel® hESC-Qualified Matrix

A soluble basement membrane extract that supports the feeder-independent expansion of human ES and iPS cells

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Corning® Matrigel® hESC-Qualified Matrix

A soluble basement membrane extract that supports the feeder-independent expansion of human ES and iPS cells

5 mL
Catalog #07181

Required Products

Overview

Corning® Matrigel® hESC-Qualified Matrix (Corning Catalog #354277) is a soluble basement membrane extract of the Engelbreth-Holm-Swarm tumor. It gels at room temperature to form a genuine reconstituted basement membrane rich in extracellular matrix proteins such as laminin, collagen IV, entactin, and heparan sulfate proteoglycan (Bissell et al.; Kleinman et al. 1982 & 1986). Growth factors, collagenases, plasminogen activators, and other undefined components have also been identified in this matrix (McGuire & Seeds; Vukicevic et al.). After dilution, the matrix is used to coat tissue culture-treated cultureware for supporting the feeder-independent expansion of human embryonic stem (ES) and induced pluripotent stem (iPS) cells.

Corning® Matrigel® hESC-Qualified Matrix is an optimized surface for stem cell research and has been widely accepted as an alternative substrate to feeder cells for the culture of human ES and iPS cells (Drukker et al.; Hammerick et al.; Xu et al. 2001 & 2004; Ludwig et al. 2006a & 2006b). It has been qualified to be compatible with mTeSR™1 (Catalog #85850), eliminating the need for time-consuming screening, while providing the reproducibility and consistency essential for human pluripotent stem cell (hPSC) research.

Coupled with feeder-free hPSC maintenance media such as mTeSR™1, mTeSR™ Plus (Catalog #05825), TeSR™-E8™ (Catalog #05990), or TeSR™2 (Catalog #05860), Corning® Matrigel® hESC-Qualified Matrix can successfully maintain human ES and iPS cell lines in the undifferentiated state. These cells retain characteristic hPSC morphology and expression of undifferentiated cell markers such as OCT-3/4, SSEA-3, and TRA-1-60. Corning® Matrigel® hESC-Qualified Matrix is certified to be free of lactose dehydrogenase elevating virus (LDEV/LDHV).

For the lot-specific dilution factor, refer to the Matrigel® Certificate of Analysis, available at www.stemcell.com/coa.
Cell Type:
Endoderm, PSC-Derived; Pluripotent Stem Cells
Species:
Human; Mouse; Rat; Non-Human Primate; Other
Brand:
Matrigel

Scientific Resources

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Publications

(8)
Nature biotechnology 2012 may

Isolation of primitive endoderm, mesoderm, vascular endothelial and trophoblast progenitors from human pluripotent stem cells.

M. Drukker et al.

Abstract

To identify early populations of committed progenitors derived from human embryonic stem cells (hESCs), we screened self-renewing, BMP4-treated and retinoic acid-treated cultures with >400 antibodies recognizing cell-surface antigens. Sorting of >30 subpopulations followed by transcriptional analysis of developmental genes identified four distinct candidate progenitor groups. Subsets detected in self-renewing cultures, including CXCR4(+) cells, expressed primitive endoderm genes. Expression of Cxcr4 in primitive endoderm was confirmed in visceral endoderm of mouse embryos. BMP4-induced progenitors exhibited gene signatures of mesoderm, trophoblast and vascular endothelium, suggesting correspondence to gastrulation-stage primitive streak, chorion and allantois precursors, respectively. Functional studies in vitro and in vivo confirmed that ROR2(+) cells produce mesoderm progeny, APA(+) cells generate syncytiotrophoblasts and CD87(+) cells give rise to vasculature. The same progenitor classes emerged during the differentiation of human induced pluripotent stem cells (hiPSCs). These markers and progenitors provide tools for purifying human tissue-regenerating progenitors and for studying the commitment of pluripotent stem cells to lineage progenitors.
Tissue engineering. Part A 2011 feb

Elastic properties of induced pluripotent stem cells.

K. E. Hammerick et al.

Abstract

The recent technique of transducing key transcription factors into unipotent cells (fibroblasts) to generate pluripotent stem cells (induced pluripotent stem cells [iPSCs]) has significantly changed the stem cell field. These cells have great promise for many clinical applications, including that of regenerative medicine. Our findings show that iPSCs can be derived from human adipose-derived stromal cells (hASCs), a notable advancement in the clinical applicability of these cells. To investigate differences between two iPS cell lines (fibroblast-iPSC and hASC-iPSC), and also the gold standard human embryonic stem cell, we looked at cell stiffness as a possible indicator of cell differentiation-potential differences. We used atomic force microscopy as a tool to determine stem cell stiffness, and hence differences in material properties between cells. Human fibroblast and hASC stiffness was also ascertained for comparison. Interestingly, cells exhibited a noticeable difference in stiffness. From least to most stiff, the order of cell stiffness was as follows: hASC-iPSC, human embryonic stem cell, fibroblast-iPSC, fibroblasts, and, lastly, as the stiffest cell, hASC. In comparing hASC-iPSCs to their origin cell, the hASC, the reprogrammed cell is significantly less stiff, indicating that greater differentiation potentials may correlate with a lower cellular modulus. The stiffness differences are not dependent on cell culture density; hence, material differences between cells cannot be attributed solely to cell-cell constraints. The change in mechanical properties of the cells in response to reprogramming offers insight into how the cell interacts with its environment and might lend clues to how to efficiently reprogram cell populations as well as how to maintain their pluripotent state.
Nature biotechnology 2006 feb

Derivation of human embryonic stem cells in defined conditions.

T. E. Ludwig et al.

Abstract

We have previously reported that high concentrations of basic fibroblast growth factor (bFGF) support feeder-independent growth of human embryonic stem (ES) cells, but those conditions included poorly defined serum and matrix components. Here we report feeder-independent human ES cell culture that includes protein components solely derived from recombinant sources or purified from human material. We describe the derivation of two new human ES cell lines in these defined culture conditions.
Nature methods 2006 aug

Feeder-independent culture of human embryonic stem cells.

T. E. Ludwig et al.

Abstract

Feeder-independent culture of human embryonic stem cells.
Stem cells (Dayton, Ohio) 2004

Immortalized fibroblast-like cells derived from human embryonic stem cells support undifferentiated cell growth.

C. Xu et al.

Abstract

Human embryonic stem cells (hESCs) have the potential to generate multiple cell types and hold promise for future therapeutic applications. Although undifferentiated hESCs can proliferate indefinitely, hESC derivatives significantly downregulate telomerase and have limited replication potential. In this study we examine whether the replicative lifespan of hESC derivatives can be extended by ectopic expression of human telomerase reverse transcriptase (hTERT), the catalytic component of the telomerase complex. To this end, we have derived HEF1 cells, a fibroblast-like cell type, differentiated from hESCs. Infection of HEF1 cells with a retrovirus expressing hTERT extends their replicative capacity, resulting in immortal human HEF1-hTERT cells. HEF1-hTERT cells can be used to produce conditioned medium (CM) capable of supporting hESC growth under feeder-free conditions. Cultures maintained in HEF1-CM show characteristics similar to mouse embryonic fibroblast CM control cultures, including morphology, surface marker and transcription factor expression, telomerase activity, differentiation, and karyotypic stability. In addition, HEF1-hTERT cells have the capacity to differentiate into cells of the osteogenic lineage. These results suggest that immortalized cell lines can be generated from hESCs and that cells derived from hESCs can be used to support their own growth, creating a genotypically homogeneous system for the culture of hESCs.
STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.