STEMdiff™ Neural Crest Differentiation Kit

Cell culture kit for establishment of hPSC-derived neural crest cells
STEMdiff™ Neural Crest Differentiation Kit

The STEMdiff™ Neural Crest Differentiation Kit efficiently generates a monolayer of Neural Crest cells.

Catalog # 08610
329 USD

Overview

STEMdiff™ Neural Crest Differentiation Kit creates a serum-free medium for differentiation of human embryonic stem (ES) cells and induced pluripotent stem (iPS) cells to neural crest cells. These neural crest cells, which are characterized by neural crest markers such as SOX10 and CD271, can be differentiated to several downstream derivatives including chondrocytes, osteoblasts, and peripheral neurons. This medium is compatible with human ES and iPS cells maintained in either mTeSR™1 (Catalog #85850), mTeSR™ Plus (Catalog #05825) or TeSR™-E8™ (Catalog #05990).
Advantages
• RAPID - Generates neural crest cells in an easy-to-use monolayer protocol in only six days
• EFFICIENT - Greater than 70% purity of multipotent neural crest cells
• MULTIPOTENT - Produces cells capable of downstream differentiation, including to peripheral neurons, chondrocytes, and osteoblasts
• VERSATILE - Compatible with human ES and iPS cells maintained in mTeSR™1, mTeSR™ Plus, or TeSR™-E8™
Components
  • STEMdiff™ Neural Induction Medium, 250 mL (Catalog #05835)
  • STEMdiff™ Neural Crest Differentiation Supplement, 0.5 mL
Subtype
Specialized Media
Cell Type
Neural Cells, PSC-Derived, Neural Stem and Progenitor Cells, Neurons, Pluripotent Stem Cells
Species
Human
Application
Cell Culture, Differentiation, Toxicity Assay
Brand
STEMdiff
Area of Interest
Disease Modeling, Drug Discovery and Toxicity Testing, Neuroscience, Stem Cell Biology
Formulation
Serum-Free

Related Products

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
STEMdiff™ Neural Crest Differentiation Kit
Catalog #
08610
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
STEMdiff™ Neural Crest Differentiation Kit
Catalog #
08610
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
STEMdiff™ Neural Crest Differentiation Kit
Catalog #
08610
Lot #
All
Language
English

Educational Materials(5)

Brochure
hPSC-Derived Neural Cell Research
Brochure
STEMCELL Neural Product Portfolio
Wallchart
Derivation and Applications of Human Pluripotent Stem Cells
Webinar
54:21
hPSC-Derived Neural Crest: Induction and Axial Patterning
Scientific Poster
Efficient Generation of Human Pluripotent Stem Cells to Neural Crest Cells

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Figure 1.

Neural crest cells are produced using STEMdiff™ Neural Crest Differentiation Kit after 6 days in culture. For information on continued passage of neural crest cells, contact us at techsupport@stemcell.com

STEMdiff™ Neural Crest Differentiation Kit Generates a Highly Pure Population of NCCs with Minimal CNS-type Progenitors

Figure 2. STEMdiff™ Neural Crest Differentiation Kit Generates a Highly Pure Population of NCCs with Minimal CNS-type Progenitors

After 6 days in culture, neural crest cells (NCCs; SOX10+, red; CD271+, light blue) outnumber CNS-type progenitors (PAX6+, green). (A) Channel merge of cells fixed 2 days after being passaged on day 6. Individual immunofluorescence channels show (B) DAPI, (C) PAX6, (D) SOX10, and (E) CD271. Scale bar = 100 μm.

NCCs Generated with the STEMdiff™ Neural Crest Differentiation Kit Are Multipotent

Figure 3. NCCs Generated with the STEMdiff™ Neural Crest Differentiation Kit Are Multipotent

NCCs (A) were cultured for 6 days and display typical morphology. (B) Culturing NCCs using established protocols generates peripheral neurons (Peripherin, green; BRN3a, red; DAPI, blue). (C) Passaging NCCs into MesenCult™-ACF Plus Medium and then into the MesenCult™-ACF Chondrogenic Differentiation Kit generates a chondrocyte pellet (Alcian Blue, Nuclear Fast Red) with deposition of cartilage around the cells. (D) Passaging NCCs into MesenCult™-ACF Plus Medium and then into the MesenCult™ Osteogenic Differentiation Kit (Human) generates an osteoblast culture with high levels of alkaline phosphatase-positive mineral deposition. Scale bar = 500 μm (A-C), 1 mm (D).

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