STEMdiff™ Dorsal Forebrain Organoid Differentiation Kit

Cell culture medium kit for robust generation of dorsal forebrain neural organoids from human pluripotent stem cells
STEMdiff™ Dorsal Forebrain Organoid Differentiation Kit

Cell culture medium kit for robust generation of dorsal forebrain neural organoids from human pluripotent stem cells

1 Kit
Catalog # 08620
548 USD
Required Products
  1. AggreWell™ Rinsing Solution
    Anti-Adherence Rinsing Solution

    Rinsing solution for cultureware to prevent cell adhesion

  2. 37μm Reversible Strainers|27215,27250
    Reversible Strainers

    Reversible strainer for filtration of single cells and isolation of cellular aggregates, including embryoid bodies and spheroids

  3. AggreWell™800 24-well plate|34811
    AggreWell™800

    Microwell culture plates for easy and reproducible production of embryoid bodies and spheroids

  4. STEMdiff™ Neural Organoid Maintenance Kit
    STEMdiff™ Neural Organoid Maintenance Kit

    Cell culture medium kit for long-term maintenance and maturation of patterned neural organoids

Overview

Robustly generate three-dimensional, patterned brain organoid cultures from human pluripotent stem cells without matrix embedding. STEMdiff™ Dorsal and Ventral Forebrain Organoid Differentiation Kits are serum-free cell culture media that work with AggreWell™-generated embryoid bodies to prevent organoid fusion and enable the scalable generation of over 500 highly reproducible organoids per kit. Adapted from protocols by Sergiu Paşca (F Birey et al. Nature, 2017), these brain-region-specific organoids are three-dimensional in vitro models with a cellular composition and structural organization that is representative of the developing human forebrain. STEMdiff™ Dorsal Forebrain Organoid Differentiation Kit (Catalog #08620) generates tissue of the early developing dorsal pallium, while STEMdiff™ Ventral Forebrain Organoid Differentiation Kit (Catalog #08630) generates tissue of the early developing ventral subpallium. Organoids generated with these kits can also be co-cultured as assembloids to study brain region interactions (F Birey et al. Nature, 2017). For extended periods of organoid culture (> 50 days), the components required for organoid maintenance are available as STEMdiff™ Neural Organoid Maintenance Kit (Catalog #100-0120).
Advantages
⦁ Reduce handling and media waste with fusion-free growth media
⦁ Obtain greater analytic sensitivity for disease phenotypes with reproducible morphology between lines and individual organoids
⦁ Generate over 500 organoids per kit using AggreWell™800 for higher-powered statistical replicates and more detailed longitudinal study
⦁ Eliminate matrix embedding steps with the matrix-free formulation and protocol
⦁ Easily process and section consistently sized organoids
⦁ Achieve long-term culture survival and reduced caspase-3 expression for neurotoxicity and neurodegenerative models
⦁ Combine modular region-patterned organoids to generate advanced assembloids for disease modeling and regenerative applications
Components
  • STEMdiff™ Neural Organoid Basal Medium 1, 20 mL
  • STEMdiff™ Neural Organoid Basal Medium 2, 500 mL
  • STEMdiff™ Neural Organoid Supplement A, 10 mL
  • STEMdiff™ Neural Organoid Supplement B, 0.25 mL
  • STEMdiff™ Neural Organoid Supplement C, 0.25 mL
Subtype
Specialized Media
Cell Type
Neural Cells, PSC-Derived, Neural Stem and Progenitor Cells, Pluripotent Stem Cells
Species
Human
Application
Cell Culture, Characterization, Differentiation, Functional Assay, Immunofluorescence, Organoid Culture, Phenotyping, Spheroid Culture
Brand
STEMdiff
Area of Interest
Disease Modeling, Drug Discovery and Toxicity Testing, Neuroscience, Organoids
Formulation
Serum-Free

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
STEMdiff™ Dorsal Forebrain Organoid Differentiation Kit
Catalog #
08620
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
STEMdiff™ Dorsal Forebrain Organoid Differentiation Kit
Catalog #
08620
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
STEMdiff™ Dorsal Forebrain Organoid Differentiation Kit
Catalog #
08620
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
STEMdiff™ Dorsal Forebrain Organoid Differentiation Kit
Catalog #
08620
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
STEMdiff™ Dorsal Forebrain Organoid Differentiation Kit
Catalog #
08620
Lot #
All
Language
English
Document Type
Safety Data Sheet 5
Product Name
STEMdiff™ Dorsal Forebrain Organoid Differentiation Kit
Catalog #
08620
Lot #
All
Language
English

Educational Materials (18)

Brochure
STEMCELL Neural Product Portfolio
Brochure
hPSC-Derived Neural Cell Research
Brochure
Organoids
Technical Bulletin
Cryogenic Tissue Processing and Section Immunofluorescence of Cerebral Organoids
Protocol
How to Dissociate 3D Neural Organoids into a Single-Cell Suspension
Wallchart
Derivation and Applications of Human Pluripotent Stem Cells
Wallchart
Cell-Reprogramming Technology and Neuroscience
Wallchart
Building Three-Dimensional Human Brain Organoids
Webinar
iPSCs As Models, Part 2: Modeling the Human Brain with Organoids
33:07
iPSCs As Models, Part 2: Modeling the Human Brain with Organoids
Webinar
ISSCR Innovation Showcase: Advanced Brain Organoid Co-Culture Systems
1:01:37
ISSCR Innovation Showcase: Advanced Brain Organoid Co-Culture Systems
Webinar
Spotlight on Organoids: Expert Panel
Webinar
Building Brain Organoids and AssemBloids™ to Study Human Development and Disease
1:02:03
Building Brain Organoids and AssemBloids™ to Study Human Development and Disease
Webinar
Challenges in Translating iPSC Technology
41:47
Challenges in Translating iPSC Technology
Webinar
Single-Cell Characterization of Dorsal and Ventral Forebrain Organoid Models
23:34
Single-Cell Characterization of Dorsal and Ventral Forebrain Organoid Models
Webinar
Nature Research Round Table: The Promise of Organoid Medicine
17:17
Nature Research Round Table: The Promise of Organoid Medicine
Webinar
hPSC Quality: Essential Considerations for Gene Editing, Cloning, Maintenance and Disease Modeling
50:49
hPSC Quality: Essential Considerations for Gene Editing, Cloning, Maintenance and Disease Modeling
Scientific Poster
A Reliable, Efficient, and Feeder-Free Method to Generate Brain-Region-Specific Dorsal and Ventral Forebrain Organoids From Human Pluripotent Stem Cells to Model Early Human Brain Development
Scientific Poster
Single-Cell RNA Sequencing Analysis of Regionally Patterned Human Pluripotent Stem Cell-Derived Neural Organoids
Load More Educational Materials

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Schematic protocol diagram to generate patterned dorsal forebrain organoids from iPSCs

Figure 1. Schematic for the STEMdiff™ Dorsal Forebrain Organoid Differentiation Kit

Human ES or iPS cell-derived dorsal forebrain organoids can be generated in 43 days. Embryoid bodies can be created in 6 days with AggreWell™800 plates. The EBs are then cultured in suspension, allowing growth and subsequent patterning to the dorsal forebrain. For the long-term maintenance and further maturation of dorsal forebrain organoids, see the PIS.

Figure 2. Fluorescent Imaging in BrainPhys™ Imaging Optimized Medium Improves Signal-to-Background Ratios of 3D Neural Cultures

GFP-labeled ventral forebrain organoids were co-cultured and fused with unlabeled dorsal forebrain organoids for one week prior to live imaging in Forebrain Organoid Differentiation Medium from STEMdiff™ Dorsal Forebrain Organoid Differentiation Kit (right) or BrainPhys™ Imaging Optimized Medium (BPI, left). Interneuron migration can be visualized more clearly in BPI. Adapted from Zabolocki et al. 2020, Nature Communications, available under a Creative Commons 4.0 License.

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