hPSC-Derived Neural Cell Research
After neural induction, human pluripotent stem cell (hPSC)-derived neural progenitor cells (NPCs) can be further differentiated into the major neural cell types of the central nervous system: neurons, astrocytes and oligodendrocytes. See MoreThese specialized neural cell types hold great promise for studying human neurological development and disease. After using STEMdiff™ neural induction protocols to generate NPCs, you can use cell type-specific STEMdiff™ differentiation and maturation kits to differentiate forebrain-type neurons, midbrain neurons, or astrocytes.
A two-step differentiation and maturation approach allows for increased overall efficiency and experimental flexibility. First, STEMdiff™ differentiation kits for forebrain-type neurons, midbrain-type neurons or astrocytes generate lineage-restricted precursors from NPCs. Researchers can then mature these precursors to functional forebrain-type neurons, midbrain- neurons or astrocytes, using the corresponding STEMdiff™ maturation kits. Many of our maturation kits have a basal medium based on BrainPhys™ Neuronal Medium, a serum-free physiological medium that is based on the formulation from the laboratory of Fred H. Gage (C Bardy et al. Proc Natl Acad Sci USA, 2015) and supports neuronal activity. hPSC-derived neuronal cultures show activity-dependent maturation, expressing more synaptically-associated proteins (e.g. J Ruden et al. Front Mol Neurosci, 2021) and a wider range of adult protein isoforms (e.g. L Miguel et al. Stem Cell Res, 2019) when cultured in BrainPhys™-based media—potentially uncovering crucial information about their complex phenotypes.
Downstream of neural crest induction, researchers can further differentiate hPSC-derived neural crest cells (NCCs) to cell types of the peripheral nervous system. NCCs generated using the STEMdiff™ Neural Crest Differentiation Kit differentiate to sensory neurons using a two-step approach with STEMdiff™ Sensory Neuron Differentiation and Maturation Kits.
For a flexible approach to generating many different neural cell subtypes according to published protocols, you may combine neural supplements, such as NeuroCult™ SM1 Neuronal Supplement (based on the published B-27 formulation) and N2 Supplement A or B , with or and a suitable basal medium, such as . Compared to traditional medium formulations, BrainPhys™ Neuronal Medium is more representative of the CNS extracellular environment, which results in hPSC-derived neuronal cultures with increased proportions of synaptically active neurons.
If 3D models are more appropriate for your experiments, you can differentiate organoids containing various neural cell types arranged in a 3D tissue structure directly from hPSCs with STEMdiff™ neural organoid kits. With options that enable developmental self-organization or patterning to particular nervous system subregions, neural organoids are powerful tools to study human developmental processes and complex cell interactions.
- Tech Tip Designing Your Neural Induction and Differentiation Workflow
- Brochure BrainPhys™ Neuronal Medium
- Webinar Modeling Neurodegenerative Diseases with STEMdiff™ hPSC-Derived Neural Culture Systems
- Webinar The Road to Functional Human Neuronal Circuits in Vitro
- Brochure hPSC-Derived Neural Cell Research
Cell Culture Media and Supplements 4 (8)
BrainPhys™ Primary Neuron KitKit for serum-free culture of primary tissue-derived neurons in BrainPhys™ Neuronal Medium
BrainPhys™ Neuronal Medium and SM1 KitKit including BrainPhys™ Neuronal Medium and SM1 Neuronal Supplement for serum-free culture of primary and ES/iPS cell-derived neurons
BrainPhys™ Neuronal MediumSerum-free neurophysiological basal medium for improved neuronal function
BrainPhys™ Without Phenol RedSerum-free neurophysiological basal medium for improved neuronal function
Cell Dyes and Detection Assay Kits 4 (2)
NeuroFluor™ NeuOMembrane-permeable fluorescent probe for the detection of live neurons
Trypan BlueReagent for counting viable mammalian cells
Tissue and Cell Culture Dissociation Reagents 4 (1)
Antibodies 4 (3)
Anti-Human Beta-Tubulin III Antibody, Clone 2G10-TB3Mouse monoclonal IgG2a antibody against human, mouse, rat betatubulin III
Anti-GFAP Antibody, Clone 2E1.E9Mouse monoclonal IgG2b antibody against human, mouse, rat GFAP (glial fibrillary acidic protein)
Anti-Beta-Tubulin III Antibody, Clone AA10Mouse monoclonal IgG2a antibody against human, mouse, rat betatubulin III
Cytokines and Proteins 4 (1)
Small Molecules 4 (6)
SIS3Activin/NODAL/TGF-β pathway inhibitor; Inhibits SMAD3
Compound ENotch pathway inhibitor; Inhibits Notch receptor and amyloid precursor protein
Dibutyryl-cAMPcAMP pathway activator; Activates cAMP-dependent protein kinases
XAV939WNT pathway inhibitor; Inhibits TNKS1 and TNKS2