Human Recombinant Flt3/Flk-2 Ligand (CHO-expressed)

Fms-like tyrosine kinase 3/fetal liver kinase-2

Human Recombinant Flt3/Flk-2 Ligand (CHO-expressed)

Fms-like tyrosine kinase 3/fetal liver kinase-2

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Overview

Flt3/Flk-2 (Fms-like tyrosine kinase 3/fetal liver kinase-2) Ligand is a hematopoietic cytokine that plays an important role as a co-stimulatory factor in the proliferation, differentiation, and survival of hematopoietic stem and progenitor cells and in the development of the immune system (Hannum et al. Nature, 1994). Flt3/Flk-2 Ligand, together with stem cell factor and thrombopoietin, is commonly used to promote expansion of primitive CD34+ hematopoietic cells in culture. In combination with myeloid cytokines such as GM-CSF, G-CSF, or M-CSF, it enhances the growth and numbers of clonogenic myeloid progenitor cells. In synergy with IL-3, IL-4, IL-7, IL-11, IL-12, IL-15, and GM-CSF and TNF-α, Flt3/Flk-2 Ligand regulates the development of lymphoid progenitor cells, including dendritic cell, B cell, T cell, and NK cell progenitors. In contrast, Flt3/Flk-2 Ligand has no significant effect on erythropoiesis or megakaryopoiesis (Drexler & Quentmeier. Growth Factors, 2004; Wodnar-Filipowicz. News Physiol, 2003).

Flt3/Flk-2 Ligand exists as membrane-bound and soluble isoforms. Both isoforms are biologically active and signal through the class III tyrosine kinase receptor (Flt3/Flk-2, CD135; Drexler & Quentmeier. Growth Factors, 2004). Flt3/Flk-2 Ligand is produced by a variety of cell types, including uncommitted and committed hematopoietic cells and stromal fibroblasts, whereas the Flt3/Flk-2 receptor is expressed on CD34+ hematopoietic stem and progenitor cells, leukemic cells, and in the brain, placenta, and testis (Drexler & Quentmeier. Growth Factors, 2004; Hannum et al. Nature, 1994).
Subtype
Cytokines
Alternative Names
FL, FLT3L, Flt3-L, Flt3 Ligand, Fms-like tyrosine kinase 3 ligand
Cell Type
Dendritic Cells, Hematopoietic Stem and Progenitor Cells, Mesoderm, PSC-Derived, NK Cells
Species
Human
Area of Interest
Immunology, Stem Cell Biology
Purity
≥ 95%

Data Figures

Figure 1. Biological Activity and Molecular Mass of Human Recombinant Flt3/Flk-2 Ligand (CHO-expressed)

(A) The biological activity of Human Recombinant Flt3/Flk-2 Ligand (CHO-expressed) was tested by its ability to promote the proliferation of human AML5 cells. The EC50 is defined as the effective concentration of the growth factor at which cell proliferation is at 50% of maximum. The EC50 in the above example is ≤ 1 ng/mL. (B) 2 μg of Human Recombinant Flt3/Flk-2 Ligand (CHO-expressed) was resolved with SDS-PAGE under non-reducing (-) and reducing (+) conditions. Human Recombinant Flt3/Flk-2 Ligand has a predicted molecular mass of 18 kDa.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
100-1710, 100-1711
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
100-1710
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
100-1711
Lot #
All
Language
English