TeSR™-E6
Defined, serum-free, xeno-free medium for pluripotent stem cells
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TeSR™-E6 -
Label for TeSR™-E6 -
Label for TeSR™-E6
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Overview
TeSR™-E6 is a defined, serum- and xeno-free medium that is based on the formulation of TeSR™-E8™, but does not contain transforming growth factor β (TGF-β) or basic fibroblast growth factor (bFGF). It may be used as a basal medium for differentiation of human embryonic stem (ES) cells and induced pluripotent stem (iPS) cells, or other applications where removal of the above cytokines is desirable.
Components:
- TeSR™-E5/E6 Basal Medium, 475 mL
- TeSR™-E6 20X Supplement, 25 mL
Subtype:
Specialized Media
Cell Type:
Pluripotent Stem Cells
Species:
Human
Application:
Cell Culture; Characterization; Differentiation
Brand:
TeSR
Area of Interest:
Drug Discovery and Toxicity Testing; Stem Cell Biology
Formulation:
Serum-Free; Xeno-Free
Scientific Resources
Product Documentation
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Educational Materials
(4)
Product Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
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Publications
(1)
Cell metabolism 2016 SEP
$$-Ketoglutarate Accelerates the Initial Differentiation of Primed Human Pluripotent Stem Cells.
Abstract
Abstract
Pluripotent stem cells (PSCs) can self-renew or differentiate from naive or more differentiated, primed, pluripotent states established by specific culture conditions. Increased intracellular $$-ketoglutarate ($$KG) was shown to favor self-renewal in naive mouse embryonic stem cells (mESCs). The effect of $$KG or $$KG/succinate levels on differentiation from primed human PSCs (hPSCs) or mouse epiblast stem cells (EpiSCs) remains unknown. We examined primed hPSCs and EpiSCs and show that increased $$KG or $$KG-to-succinate ratios accelerate, and elevated succinate levels delay, primed PSC differentiation. $$KG has been shown to inhibit the mitochondrial ATP synthase and to regulate epigenome-modifying dioxygenase enzymes. Mitochondrial uncoupling did not impede $$KG-accelerated primed PSC differentiation. Instead, $$KG induced, and succinate impaired, global histone and DNA demethylation in primed PSCs. The data support $$KG promotion of self-renewal or differentiation depending on the pluripotent state.
This product is sold for research use only (whether the buyer is an academic or for-profit entity) under a non-transferable, limited-use license. Purchase of this product does not include the right to sell, use or otherwise transfer this product for commercial purposes (i.e., any activity undertaken for consideration, such as use of this product for manufacturing, or resale of this product or any materials made using this product, or use of this product or any materials made using this product to provide services) or clinical use (i.e., administration of this product or any material using this product to humans) or the right to implant any material made using this product into an animal by, or in collaboration with, a for-profit entity, for purposes other than basic pre-clinical research applications (including without limitation teratoma assays) to validate the function of the cells. Purchasers wishing to use the product for purposes other than research use should contact Geron Corporation’s Business Development office at (650) 473-7700 or corpdev@geron.com. Purchasers who do not agree to the terms and conditions set forth above should return the product in acceptable conditions to the seller for a refund.
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.