Flt3/Flk-2 Ligand exists as membrane-bound and soluble isoforms. Both isoforms are biologically active and signal through the class III tyrosine kinase receptor (Flt3/Flk-2, CD135; Rosnet et al.). Flt3/Flk-2 Ligand is produced by a variety of cell types, including uncommitted and committed hematopoietic cells and stromal fibroblasts, whereas expression of the Flt3/Flk-2 receptor is restricted to CD34+ hematopoietic stem and progenitor cells. Flt3/Flk-2 receptor is also expressed on leukemic cells and outside the hematopoietic system in the brain, placenta, and testis (Drexler & Quentmeier; Hannum et al.). This product is animal component-free.
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Data and Publications
(A) The biological activity of Human Recombinant Flt3/Flk-2 Ligand, ACF was tested by its ability to promote the proliferation of OCI-AML5
cells. Cell proliferation was measured using a fluorometric assay method. The EC50 is defined as the effective concentration of the
cytokine at which cell proliferation is at 50% of maximum. The EC50 in the example above is 0.498 ng/mL.
(B) 1 μg of Human Recombinant Flt3/Flk-2 Ligand, ACF was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant Flt3/Flk-2 Ligand, ACF has a predicted molecular mass of 17.6 kDa.