Human Recombinant bFGF

Basic fibroblast growth factor
Catalog #
78003_C
Basic fibroblast growth factor
From: 82 USD

Overview

Basic fibroblast growth factor (bFGF) is a prototypic member of the fibroblast growth factor family. Cytokines in the FGF family possess broad mitogenic and cell survival activities (Folkman & Klagsbrun; Kimelman & Kirschner) and are involved in a variety of biological processes including cell proliferation, differentiation, survival, and apoptosis (Folkman & Klagsbrun; Klagsbrun; Rifkin & Moscatelli). bFGF has the β-trefoil structure (Ponting & Russell), binds to the four FGF receptor (FGFR) family members, and activates JAK/STAT, PI3K, ERK1/2, and other receptor tyrosine kinase (RTK) signaling pathways. It supports the maintenance of undifferentiated human embryonic stem cells (Xu et al.; Kang et al.), stimulates human embryonic stem cells to form neural rosettes (Zhang et al.), and improves proliferation of human mesenchymal stem cells and enhances chondrogenic differentiation (Solchaga et al.).
Subtype
Cytokines, Growth Factors
Alternative Names
Basic fibroblast growth factor, FGF-β, FGF2, Fibroblast growth factor-basic, HBGF-2
Cell Type
Brain Tumor Stem Cells, Endoderm, PSC-Derived, Hematopoietic Stem and Progenitor Cells, Mesenchymal Stem and Progenitor Cells, Mesoderm, PSC-Derived, Neural Cells, PSC-Derived, Neural Stem and Progenitor Cells, Neurons, Pluripotent Stem Cells, Prostate Cells
Species
Human
Area of Interest
Epithelial Cell Biology, Neuroscience, Stem Cell Biology
Molecular Weight
17.1 kDa
Purity
> 95%

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
Human Recombinant bFGF
Catalog #
78003, 78003.1, 78003.2
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
Human Recombinant bFGF
Catalog #
78003, 78003.1, 78003.2
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
Human Recombinant bFGF
Catalog #
78003, 78003.1, 78003.2
Lot #
All
Language
English

Educational Materials(5)

Brochure
Primary Neuronal Culture: Standardized Media and Reagents
Brochure
BrainPhys™ Neuronal Medium for Improved Neuronal Function
Brochure
hPSC-Derived Neural Cell Research
Brochure
Cytokines, Chemokines and Growth Factors
Brochure
Products for Human Pluripotent Stem Cells

Product Applications

Data and Publications

Data

(A) The biological activity of Human Recombinant bFGF was tested by its ability to promote the proliferation of BALB/c 3T3 cells. Cell proliferation was measured using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which cell proliferation is at 50% of maximum. The EC50 in the above example is 0.1 ng/mL.
(B) 2 μg of Human Recombinant bFGF was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant bFGF has a predicted molecular mass of 17.1 kDa.

Publications (1)

Experimental hematology 2011 AUG Retinoblastoma-binding proteins 4 and 9 are important for human pluripotent stem cell maintenance. O'Connor MD et al.

Abstract

OBJECTIVE: The molecular mechanisms that maintain human pluripotent stem (PS) cells are not completely understood. Here we sought to identify new candidate PS cell regulators to facilitate future improvements in their generation, expansion, and differentiation. MATERIALS AND METHODS: We used bioinformatic analyses of multiple serial-analysis-of-gene-expression libraries (generated from human PS cells and their differentiated derivatives), together with small interfering RNA (siRNA) screening to identify candidate pluripotency regulators. Validation of candidate regulators involved promoter analyses, Affymetrix profiling, real-time PCR, and immunoprecipitation. RESULTS: Promoter analysis of genes differentially expressed across multiple serial-analysis-of-gene-expression libraries identified E2F motifs in the promoters of many PS cell-specific genes (e.g., POU5F1, NANOG, SOX2, FOXD3). siRNA analyses identified two retinoblastoma binding proteins (RBBP4, RBBP9) as required for maintenance of multiple human PS cell types. Both RBBPs were bound to RB in human PS cells, and E2F motifs were present in the promoters of genes whose expression was altered by decreasing RBBP4 and RBBP9 expression. Affymetrix and real-time PCR studies of siRNA-treated human PS cells showed that reduced RBBP4 or RBBP9 expression concomitantly decreased expression of POU5F1, NANOG, SOX2, and/or FOXD3 plus certain cell cycle genes (e.g., CCNA2, CCNB1), while increasing expression of genes involved in organogenesis (particularly neurogenesis). CONCLUSIONS: These results reveal new candidate positive regulators of human PS cells, providing evidence of their ability to regulate expression of pluripotency, cell cycle, and differentiation genes in human PS cells. These data provide valuable new leads for further elucidating mechanisms of human pluripotency.
View All Publications

Contact STEMCELL Technologies

Our Customer Service, Sales, and Product and Scientific Support departments in North America are available between 6 am and 5 pm Pacific Time (9 am and 8 pm Eastern Time). One of our representatives will be happy to help you by telephone or email. Please complete the form to contact us by email. A representative will get back to you shortly.
  •  

StemCell Technologies Inc. and affiliates ("STEMCELL Technologies") does not share your email address with third parties. StemCell Technologies Inc. will use your email address to confirm your identity and send you newsletters, transaction-related emails, promotional and customer service emails in accordance with our privacy policy. You can change your email preferences at any time.