GloCell™ Fixable Viability Dye Red 710
Amine-labeling fluorescent dye for live/dead staining of mammalian cells
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GloCell™ Fixable Viability Dye Red 710 -
Label for GloCell™ Fixable Viability Dye Red 710
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Required Products
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Cell separation buffer -
Dulbecco’s phosphate-buffered saline without calcium and magnesium
Overview
GloCell™ Fixable Viability Dye Red 710 is a fluorescent cell viability dye for staining live/dead mammalian cells in applications such as flow cytometry. The dye irreversibly binds intracellular and cell surface amine groups and can be used prior to fixation, permeabilization, or cryopreservation. Cells with compromised plasma membranes become permeable to the GloCell™ dye, resulting in greater fluorescence compared to live cells in a cell viability assay.
GloCell™ Fixable Viability Dye Red 710 is excited by the red laser (633 nm) with an excitation maximum of 647 nm, and it has a fluorescence emission maximum of 710 nm that can be detected using the 710/50 band pass filter used to detect Alexa Fluor® 700.
GloCell™ Fixable Viability Dye Red 710 is excited by the red laser (633 nm) with an excitation maximum of 647 nm, and it has a fluorescence emission maximum of 710 nm that can be detected using the 710/50 band pass filter used to detect Alexa Fluor® 700.
Application:
Flow Cytometry; Immunofluorescence
Brand:
GloCell
Area of Interest:
Angiogenic Cell Research; Antibody Development; Cancer Research; Cell Line Development; Chimerism; Disease Modeling; Drug Discovery and Toxicity Testing; Endothelial Cell Biology; Epithelial Cell Biology; HIV; HLA; Hybridoma Generation; Immunology; Infectious Diseases; Metabolism; Mitosis; Neuroscience; Organoids; Respiratory Research; Stem Cell Biology; Cell Therapy; Transplantation Research
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This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
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Figure 1.
(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with GloCell™ Fixable Viability Dye Red 710. (B) Flow cytometry analysis of human multiple myeloma bone marrow mononuclear cells (BMMCs) labeled with GloCell™ Fixable Viability Dye Red 710.
Figure 2.
Excitation/Emission spectra for GloCell™ Fixable Viability Dye Red 710. Excitation max: 693 nm, Emission Max: 713 nm
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.