Hematopoietic Stem and Progenitor Cell Research

A multipotent hematopoietic stem cell (HSC) is defined by the properties of self-renewal and differentiation into all of the different types of mature blood cells. See MoreAs HSCs divide, they progress through a series of lineage commitment steps, producing hematopoietic progenitor cells (HPCs) that are shorter lived and more restricted in their differentiation potential.

Whether you’re uncovering the mysteries of HSC biology, developing new cell therapies or manipulating cells with CRISPR-Cas9, the culture conditions you choose can make a difference in your experiments. Our serum-free media and premixed cytokine supplements provide optimized, highly reproducible culture conditions for your research.

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Brochure Small Molecules for HSPC Expansion: UM171 and UM729
Brochure StemSpan™ CD34+ Expansion Supplement
Brochure StemSpan™ Myeloid Expansion Supplement
Brochure StemSpan™ Erythroid Expansion Supplement
Brochure StemSpan™ Megakaryocyte Expansion Supplement

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Products

Cell Culture Media and Supplements (4 8)

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StemSpan™ SFEM

Serum-free medium for culture and expansion of hematopoietic cells
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Iscove's Modified Dulbecco's Medium

Iscove's Modified Dulbecco's Medium (IMDM) with 25 mM HEPES
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DMEM/F-12 with 15 mM HEPES

Dulbecco's Modified Eagle's Medium/Nutrient Ham's Mixture F-12 (DMEM/F-12) with 15 mM HEPES buffer
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DMEM with 4500 mg/L D-Glucose

Dulbecco's Modified Eagle's Medium (DMEM) with 4500 mg/L D-glucose

Small Molecules (4 14)

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LY2228820

p38 MAP kinase (MAPK) inhibitor
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D4476

Activin/NODAL/TGF-β pathway inhibitor; Inhibits CK1, ALK5 and p38 MAPK
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GANT 58

Hedgehog pathway inhibitor; Inhibits GLI1
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BMS 493

Retinoid pathway activator; Activates pan-retinoic acid receptor

Cytokines and Proteins (4 28)

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Human/Mouse Recombinant FGF-8B, ACF

Fibroblast growth factor 8B, animal component-free
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Mouse Recombinant GM-CSF (CHO-expressed)

Granulocyte-macrophage colony-stimulating factor
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Mouse Recombinant PDGF-BB

Platelet-derived growth factor BB
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Human/Mouse Recombinant BMP-2, ACF

Bone morphogenetic protein 2, animal component-free

Cultureware and General Supplies (4 7)

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Falcon® 96-Well Flat-Bottom Microplate, Tissue Culture-Treated

Clear polystyrene, flat-bottom, tissue culture-treated multiwell plate with lid
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Falcon® 24-Well Flat-Bottom Plate, Tissue Culture-Treated

Clear polystyrene flat-bottom, tissue culture-treated multiwell plate with lid
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Corning® 245 mm Square Dish, Non-Treated

Square dish without handles
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Corning® Disposable Erlenmeyer Flask, 500 mL

Sterile polycarbonate baffled Erlenmeyer flask

Buffers and Solutions (4 4)

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Dulbecco's Phosphate Buffered Saline with 2% Fetal Bovine Serum

Cell culture buffer
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HBSS with 10 mM HEPES, Without Phenol Red

Hanks' Balanced Salt Solution (HBSS) modified with 10 mM HEPES, without phenol red
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HBSS, Modified (Without Ca++ and Mg++)

Hanks' Balanced Salt Solution (HBSS) without calcium and magnesium
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D-PBS (Without Ca++ and Mg++)

Dulbecco’s phosphate-buffered saline without calcium and magnesium

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