Hematopoietic Stem and Progenitor Cell Research

A multipotent hematopoietic stem cell (HSC) is defined by the properties of self-renewal and differentiation into all of the different types of mature blood cells. See MoreAs HSCs divide, they progress through a series of lineage commitment steps, producing hematopoietic progenitor cells (HPCs) that are shorter lived and more restricted in their differentiation potential.

Whether you’re uncovering the mysteries of HSC biology, developing new cell therapies or manipulating cells with CRISPR-Cas9, the culture conditions you choose can make a difference in your experiments. Our serum-free media and premixed cytokine supplements provide optimized, highly reproducible culture conditions for your research.

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Brochure Small Molecules for HSPC Expansion: UM171 and UM729
Brochure StemSpan™ CD34+ Expansion Supplement
Brochure StemSpan™ Myeloid Expansion Supplement
Brochure StemSpan™ Erythroid Expansion Supplement
Brochure StemSpan™ Megakaryocyte Expansion Supplement

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Products

Cell Culture Media and Supplements 4 (8)

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BIT 9500 Serum Substitute

Supplement for serum-free cell culture media
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MEM with Earle's & NEAA

Minimum Essential Medium Eagle (MEM) with Earle's salts and <br /> non-essential amino acids (NEAA)
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Alpha MEM without Nucleosides

Minimum Essential Medium Eagle - alpha modification (Alpha MEM) without nucleosides
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DMEM/F-12 with 15 mM HEPES

Dulbecco's Modified Eagle's Medium/Nutrient Ham's Mixture F-12 (DMEM/F-12) with 15 mM HEPES buffer

Small Molecules 4 (14)

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OAC1

Inducer of OCT4 expression
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16,16-Dimethyl Prostaglandin E2

Prostanoid pathway activator; Inhibits 15-hydroxy PGDH
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Valproic Acid (Sodium Salt)

Epigenetic modifier; Inhibits histone deacetylase (HDAC)1
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LY2228820

p38 MAP kinase (MAPK) inhibitor

Cytokines and Proteins 4 (28)

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Human/Mouse Recombinant FGF-8B, ACF

Fibroblast growth factor 8B, animal component-free
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Mouse Recombinant GM-CSF (CHO-expressed)

Granulocyte-macrophage colony-stimulating factor
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Mouse Recombinant PDGF-BB

Platelet-derived growth factor BB
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Human/Mouse Recombinant BMP-2, ACF

Bone morphogenetic protein 2, animal component-free

Cultureware and General Supplies 4 (3)

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Falcon® 96-Well Flat-Bottom Microplate, Tissue Culture-Treated

Clear polystyrene, flat-bottom, tissue culture-treated multiwell plate with lid
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Falcon® 24-Well Flat-Bottom Plate, Tissue Culture-Treated

Clear polystyrene flat-bottom, tissue culture-treated multiwell plate with lid
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Corning® Disposable Erlenmeyer Flask, 125 mL

Sterile polycarbonate baffled Erlenmeyer flask

Buffers and Solutions 4 (4)

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Dulbecco's Phosphate Buffered Saline with 2% Fetal Bovine Serum

Cell culture buffer
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HBSS, Modified (Without Ca++ and Mg++)

Hanks' Balanced Salt Solution (HBSS) without calcium and magnesium
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HBSS with 10 mM HEPES, Without Phenol Red

Hanks' Balanced Salt Solution (HBSS) modified with 10 mM HEPES, without phenol red
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D-PBS (Without Ca++ and Mg++)

Dulbecco’s phosphate-buffered saline without calcium and magnesium

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