Annexin V Apoptosis Detection Kit with 7-AAD

Apoptosis staining and detection kit

Annexin V Apoptosis Detection Kit with 7-AAD

Apoptosis staining and detection kit

From: 292 USD
Catalog #
100-0337_C
Apoptosis staining and detection kit

What's Included

  • Annexin V (Catalog #100-0329 or Catalog #100-0331 or Catalog #100-0333),1X 100 Tests
    • Annexin V Binding Buffer (Catalog #100-0334), 1 x 50 mL
    • 7-AAD (Catalog #75001), 1X 200 Tests

Overview

The Annexin V Apoptosis Detection Kit includes Annexin V (Catalog #100-0329 or Catalog #100-0331 or Catalog #100-0333), Annexin V Binding Buffer (Catalog #100-0334), and 7-AAD (Catalog #75001). The kit is used for the combined detection of early-stage cell apoptosis (using Annexin V) and late-stage cell apoptosis or necrosis (using both Annexin V and 7-AAD).
Annexin V belongs to the annexin family of intracellular proteins that bind to membrane phospholipids in a calcium-dependent manner. This dye has a high affinity for phosphatidylserine (PS) that is present in the inner leaflet of the plasma membrane. During early-stage cell apoptosis, PS is translocated from the inner to the outer leaflet of the cell membrane, exposing it to the external environment, where it is detected by Annexin V. This process of PS translocation occurs prior to the loss of membrane integrity. As cells progress through apoptosis and towards necrosis, the cell membrane is compromised and consequently the 7-AAD viability dye passes into the cell. Thus, cells undergoing early apoptosis stain positive for Annexin V and negative for viability dyes, while apoptotic death or necrosis is characterized by positive staining for both Annexin V and 7-AAD.
Conjugation
APC, FITC, PE
Application
Flow Cytometry
Area of Interest
Drug Discovery and Toxicity Testing, Immunology

Scientific Resources

Product Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
100-0337
Lot #
All
Language
English
Catalog #
100-0338
Lot #
All
Language
English
Catalog #
100-0339
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Catalog #
100-0337
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Catalog #
100-0337
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Catalog #
100-0337
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Catalog #
100-0338
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Catalog #
100-0338
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Catalog #
100-0338
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Catalog #
100-0339
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Catalog #
100-0339
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Catalog #
100-0339
Lot #
All
Language
English

Product Applications

Data and Publications

Figure showing flow cytometry analysis of C57BL/6 mouse thymocytes incubated at 37°C with 1 µM dexamethasone overnight. Cells were harvested and labeled with APC-conjugated Annexin V and 7-AAD.

Figure 1. Data for Annexin V, APC

Flow cytometry analysis of C57BL/6 mouse thymocytes incubated at 37°C with 1 µM dexamethasone overnight. Cells were harvested and labeled with APC-conjugated Annexin V and 7-AAD (Catalog #75001).

Figure showing flow cytometry analysis of C57BL/6 mouse thymocytes incubated at 37°C with 1 µM dexamethasone overnight. Cells were harvested and labeled with FITC-conjugated Annexin V and 7-AAD.

Figure 2. Data for Annexin V, FITC

Flow cytometry analysis of C57BL/6 mouse thymocytes incubated at 37°C with 1 µM dexamethasone overnight. Cells were harvested and labeled with FITC-conjugated Annexin V and 7-AAD (Catalog #75001).

Figure showing flow cytometry analysis of Con A-stimulated (3 days) C57BL/6 mouse splenocytes labeled with Anti-Mouse CD152 (CTLA-4) Antibody, Clone UC10-4F10-11, followed by a goat anti-Armenian hamster IgG antibody, FITC or an Armenian hamster IgG isotype control antibody, followed by a goat anti-Armenian hamster IgG, FITC.

Figure 3. Data for Annexin V, PE

Figure showing flow cytometry analysis of C57BL/6 mouse thymocytes incubated at 37°C with 1 µM dexamethasone overnight. Cells were harvested and labeled with PE-conjugated Annexin V and 7-AAD.

Contact STEMCELL Technologies

Our Customer Service, Sales, and Product and Scientific Support departments in North America are available between 6 am and 5 pm Pacific Time (9 am and 8 pm Eastern Time). One of our representatives will be happy to help you by telephone or email. Please complete the form to contact us by email. A representative will get back to you shortly.
  •  

StemCell Technologies Inc. and affiliates ("STEMCELL Technologies") does not share your email address with third parties. StemCell Technologies Inc. will use your email address to confirm your identity and send you newsletters, transaction-related emails, promotional and customer service emails in accordance with our privacy policy. You can change your email preferences at any time.