EasySep™ Human Whole Blood CD34 Positive Selection Kit II

Immunomagnetic positive selection kit
Catalog #
17879_C
Immunomagnetic positive selection kit
From: 612 USD
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Required Products
  1. EasySep™ Buffer
    EasySep™ Buffer

    Cell separation buffer

  2. "The Big Easy" EasySep™ Magnet
    "The Big Easy" EasySep™ Magnet

    Magnet for column-free immunomagnetic separation

Overview

The EasySep™ Human Whole Blood CD34 Positive Selection Kit II is designed to isolate CD34+ cells from fresh whole blood or freshly prepared buffy coat by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD34 and dextran-coated magnetic particles. The cocktail also contains an antibody to human Fc receptor to minimize nonspecific binding. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD34 antigen is expressed on hematopoietic stem and progenitor cells.

This product can be used instead of the EasySep™ Human Whole Blood CD34 Positive Selection Kit (Catalog #18086).

If isolating CD34+ cells from fresh cord blood, we recommend using the EasySep™ Human Cord Blood CD34 Positive Selection Kit II (Catalog #17896). Alternatively, if isolating CD34+ cells from any other sample type, the EasySep™ Human CD34 Positive Selection Kit II (Catalog #17856) should be used.
Advantages
• Fast and easy-to-use
• No columns required
Components
  • EasySep™ Human Whole Blood CD34 Positive Selection Kit II (Catalog #17879)
    • EasySep™ Human Whole Blood CD34 Positive Selection Cocktail II, 3 x 1 mL
    • EasySep™ Dextran RapidSpheres™ 50100, 3 x 1 mL
    • EasySep™ RBC Lysis Buffer 10X Concentrate, 10 mL (Catalog #20110)
  • RoboSep™ Human Whole Blood CD34 Positive Selection Kit II (Catalog #17879RF)
    • EasySep™ Human Whole Blood CD34 Positive Selection Cocktail II, 3 x 1 mL
    • EasySep™ Dextran RapidSpheres™ 50100, 3 x 1 mL
    • EasySep™ RBC Lysis Buffer 10X Concentrate, 10 mL (Catalog #20110)
    • RoboSep™ Buffer (Catalog #20104) x 2
    • RoboSep™ Filter Tips (Catalog #20125) x 2
Magnet Compatibility
• "The Big Easy" EasySep™ Magnet (Catalog #18001)
• RoboSep™-S (Catalog #21000)
Subtype
Cell Isolation Kits
Cell Type
Hematopoietic Stem and Progenitor Cells
Species
Human
Sample Source
Buffy Coat, Whole Blood
Selection Method
Positive
Application
Cell Isolation
Brand
EasySep, RoboSep
Area of Interest
Chimerism, Immunology, Stem Cell Biology

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
EasySep™ Human Whole Blood CD34 Positive Selection Kit II
Catalog #
17879
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
RoboSep™ Human Whole Blood CD34 Positive Selection Kit II
Catalog #
17879RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ Human Whole Blood CD34 Positive Selection Kit II
Catalog #
17879
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ Human Whole Blood CD34 Positive Selection Kit II
Catalog #
17879
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
EasySep™ Human Whole Blood CD34 Positive Selection Kit II
Catalog #
17879
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
EasySep™ Human Whole Blood CD34 Positive Selection Kit II
Catalog #
17879
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
RoboSep™ Human Whole Blood CD34 Positive Selection Kit II
Catalog #
17879RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
RoboSep™ Human Whole Blood CD34 Positive Selection Kit II
Catalog #
17879RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
RoboSep™ Human Whole Blood CD34 Positive Selection Kit II
Catalog #
17879RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
RoboSep™ Human Whole Blood CD34 Positive Selection Kit II
Catalog #
17879RF
Lot #
All
Language
English

Educational Materials (7)

Brochure
EasySep™ Cell Separation Technology
Brochure
Human CD34+ Cell Isolation Product Selection
Brochure
Hematopoietic Stem and Progenitor Cells - Products for Your Research
Wallchart
Human Immune Cytokines
Video
How to Isolate PBMCs from Whole Blood Using Density Gradient Centrifugation (Ficoll™ or Lymphoprep™)
1:37
How to Isolate PBMCs from Whole Blood Using Density Gradient Centrifugation (Ficoll™ or Lymphoprep™)
Video
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
1:13
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
Video
Simultaneous Cell Isolation from Multiple Samples Using the EasyEights™ EasySep™ Magnet
0:57
Simultaneous Cell Isolation from Multiple Samples Using the EasyEights™ EasySep™ Magnet

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Starting with whole blood, the CD34+ cell content of the isolated fraction is typically 90.4 ± 7.0% (gated on CD45+ cells; mean ± SD using RoboSep™-S). In the above example, the purities of the start and final isolated fractions are 0.06% and 95.31%, respectively. NOTE: RBCs were removed by lysis prior to flow cytometry.

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