EasySep™ Human Whole Blood CD34 Positive Selection Kit II

Immunomagnetic positive selection kit

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EasySep™ Human Whole Blood CD34 Positive Selection Kit II

Immunomagnetic positive selection kit

From: 707 USD
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Immunomagnetic positive selection kit
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Product Advantages


  • Fast and easy-to-use

  • No columns required

What's Included

  • EasySep™ Human Whole Blood CD34 Positive Selection Kit II (Catalog #17879)
    • EasySep™ Human Whole Blood CD34 Positive Selection Cocktail II, 3 x 1 mL
    • EasySep™ Dextran RapidSpheres™ 50100, 3 x 1 mL
    • EasySep™ RBC Lysis Buffer 10X Concentrate, 10 mL (Catalog #20110)
  • RoboSep™ Human Whole Blood CD34 Positive Selection Kit II (Catalog #17879RF)
    • EasySep™ Human Whole Blood CD34 Positive Selection Cocktail II, 3 x 1 mL
    • EasySep™ Dextran RapidSpheres™ 50100, 3 x 1 mL
    • EasySep™ RBC Lysis Buffer 10X Concentrate, 10 mL (Catalog #20110)
    • RoboSep™ Buffer (Catalog #20104) x 2
    • RoboSep™ Filter Tips (Catalog #20125) x 2
Products for Your Protocol

Overview

The EasySep™ Human Whole Blood CD34 Positive Selection Kit II is designed to isolate CD34+ cells from fresh whole blood or freshly prepared buffy coat by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD34 and dextran-coated magnetic particles. The cocktail also contains an antibody to human Fc receptor to minimize nonspecific binding. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD34 antigen is expressed on hematopoietic stem and progenitor cells.

This product can be used instead of the EasySep™ Human Whole Blood CD34 Positive Selection Kit (Catalog #18086).

If isolating CD34+ cells from fresh cord blood, we recommend using the EasySep™ Human Cord Blood CD34 Positive Selection Kit II (Catalog #17896). Alternatively, if isolating CD34+ cells from any other sample type, the EasySep™ Human CD34 Positive Selection Kit II (Catalog #17856) should be used.
Magnet Compatibility
• "The Big Easy" EasySep™ Magnet (Catalog #18001)
• RoboSep™-S (Catalog #21000)
Subtype
Cell Isolation Kits
Cell Type
Hematopoietic Stem and Progenitor Cells
Species
Human
Sample Source
Buffy Coat, Whole Blood
Selection Method
Positive
Application
Cell Isolation
Brand
EasySep, RoboSep
Area of Interest
Chimerism, Immunology, Stem Cell Biology

Data Figures

Starting with whole blood, the CD34+ cell content of the isolated fraction is typically 90.4 ± 7.0% (gated on CD45+ cells; mean ± SD using RoboSep™-S). In the above example, the purities of the start and final isolated fractions are 0.06% and 95.31%, respectively. NOTE: RBCs were removed by lysis prior to flow cytometry.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

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17879
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English
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17879RF
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English
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Safety Data Sheet 1
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17879
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English
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Safety Data Sheet 2
Catalog #
17879
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English
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Safety Data Sheet 3
Catalog #
17879
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English
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Safety Data Sheet 1
Catalog #
17879RF
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All
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English
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Safety Data Sheet 2
Catalog #
17879RF
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All
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English
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Safety Data Sheet 3
Catalog #
17879RF
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English
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Safety Data Sheet 4
Catalog #
17879RF
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All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more