STEMdiff™ Atrial Cardiomyocyte Differentiation Kit

Culture medium kit for differentiation of human PSCs to atrial cardiomyocytes
STEMdiff™ Atrial Cardiomyocyte Differentiation Kit

Culture medium kit for differentiation of human PSCs to atrial cardiomyocytes

1 Kit
Catalog # 100-0215
679 USD
Required Products
  1. Gentle Cell Dissociation Reagent
    Gentle Cell Dissociation Reagent

    cGMP, enzyme-free cell dissociation reagent

  2. Y-27632 (Dihydrochloride)
    Y-27632

    RHO/ROCK pathway inhibitor; Inhibits ROCK1 and ROCK2

  3. D-PBS Without Ca++ and Mg++
    D-PBS (Without Ca++ and Mg++)

    Dulbecco’s phosphate-buffered saline without calcium and magnesium

Overview

STEMdiff™ Atrial Cardiomyocyte Differentiation Kit is optimized for the differentiation and maintenance of human embryonic stem and induced pluripotent stem cells into atrial cardiomyocytes (cardiac troponin T-positive; cTnT+). This kit can be used to generate atrial cardiomyocytes derived from a clump culture of human pluripotent stem cells (hPSCs) maintained in mTeSR™1 (Catalog #85850), mTeSR™ Plus (Catalog #100-0276), or TeSR™-E8™ (Catalog #05990). With greater than 80% yield purity, an average of 1 x 10^6 cTnT+ cells can be harvested from a single well of a 12-well plate.

STEMdiff™ Cardiomyocyte Maintenance Kit (Catalog #05020) comprises the maintenance basal medium and supplement and allows for long-term maintenance of hPSC-derived atrial cardiomyocytes for one month or longer. These cardiomyocytes can be used in various downstream applications and analyses.
Advantages
⦁ Simple monolayer protocol, compatible with STEMdiff™ cardiomyocyte workflow
⦁ Establishment and efficient generation of up to 50 million atrial cardiomyocytes per kit
⦁ Cardiomyocytes generated with this kit express high levels of atrial-specific genes and demonstrate dominant atrial-like phenotypes including faster beat rate and decreased field potential duration when compared to those generated using the STEMdiff™ Cardiomyocyte Differentiation Kit
⦁ Maximum reproducibility with minimal variability across multiple hPSC lines

Components
  • STEMdiff™ Cardiomyocyte Differentiation Basal Medium, 380 mL
  • STEMdiff™ Atrial Cardiomyocyte Differentiation Supplement A (10X), 10 mL
  • STEMdiff™ Atrial Cardiomyocyte Differentiation Supplement B (10X), 10 mL
  • STEMdiff™ Atrial Cardiomyocyte Differentiation Supplement C (10X), 20 mL
  • STEMdiff™ Cardiomyocyte Maintenance Basal Medium, 490 mL
  • STEMdiff™ Atrial Cardiomyocyte Maintenance Supplement (50X), 10 mL
Subtype
Specialized Media
Cell Type
Cardiomyocytes, PSC-Derived
Species
Human
Application
Cell Culture, Differentiation
Brand
STEMdiff
Area of Interest
Cell Line Development, Disease Modeling, Drug Discovery and Toxicity Testing, Stem Cell Biology
Formulation
Serum-Free

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
STEMdiff™ Atrial Cardiomyocyte Differentiation Kit
Catalog #
100-0215
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
STEMdiff™ Atrial Cardiomyocyte Differentiation Kit
Catalog #
100-0215
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
STEMdiff™ Atrial Cardiomyocyte Differentiation Kit
Catalog #
100-0215
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
STEMdiff™ Atrial Cardiomyocyte Differentiation Kit
Catalog #
100-0215
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
STEMdiff™ Atrial Cardiomyocyte Differentiation Kit
Catalog #
100-0215
Lot #
All
Language
English
Document Type
Safety Data Sheet 5
Product Name
STEMdiff™ Atrial Cardiomyocyte Differentiation Kit
Catalog #
100-0215
Lot #
All
Language
English
Document Type
Safety Data Sheet 6
Product Name
STEMdiff™ Atrial Cardiomyocyte Differentiation Kit
Catalog #
100-0215
Lot #
All
Language
English

Educational Materials (5)

Brochure
STEMdiff™ Cardiomyocyte Differentiation Kit
Protocol
How to Dissociate and Plate Human Pluripotent Stem Cell-Derived Cardiomyocytes for Microelectrode Array (MEA) Assay
Webinar
Modeling Arrhythmias Using hPSC-Derived Cardiomyocytes and Tracking Their Excitability
43:45
Modeling Arrhythmias Using hPSC-Derived Cardiomyocytes and Tracking Their Excitability
Scientific Poster
Heterozygous Deletion of KCNH2 Models Long QT Type 2 in Human Pluripotent Stem Cell-Derived Cardiomyocytes
Scientific Poster
Rescue of Drug-Induced Long QT Syndrome Type 2 Using a hERG Channel Activator in Human Pluripotent Stem Cell-Derived Cardiomyocytes

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

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