Shz-1

Inducer of cardiac differentiation
Shz-1

Inducer of cardiac differentiation

10 mg
Catalog # 73422
175 USD

Overview

Shz-1 is a cell-permeable, sulphonyl hydrazone (Shz) compound that activates cardiac differentiation through induction of genes such as Nkx2.5 (Sadek et al.).

DIFFERENTIATION
· Promotes cardiac differentiation in mouse induced pluripotent stem cells (Quattrocelli et al.).
·Induces cardiac differentiation of human mobilized peripheral blood mononuclear cells; these cells enhance myocardial regenerative repair when injected in a cryo-injured rat heart model (Sadek et al.).
Alternative Names
Sulfonylhydrazone-1
Cell Type
Cardiomyocytes, PSC-Derived, Pluripotent Stem Cells
Species
Human, Mouse, Rat, Non-Human Primate, Other
Application
Differentiation
Area of Interest
Stem Cell Biology
CAS Number
326886-05-9
Chemical Formula
C₁₃H₁₁BrN₂O₃S
Molecular Weight
355.2 g/mol
Purity
≥ 98%

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
Shz-1
Catalog #
73422
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Shz-1
Catalog #
73422
Lot #
All
Language
English

Educational Materials (3)

Brochure
Small Molecules for Stem Cell Research
Brochure
Small Molecules for Cancer Research
Mini Review
Small Molecules

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Publications (2)

Journal of cellular biochemistry 2011 Synthetic sulfonyl-hydrazone-1 positively regulates cardiomyogenic microRNA expression and cardiomyocyte differentiation of induced pluripotent stem cells. Quattrocelli M et al.

Abstract

Induced pluripotent stem cells (iPSCs) are obtained from adult cells through overexpression of pluripotency factors. iPSCs share many features with embryonic stem cells (ESCs), circumventing ethical issues, and, noteworthy, match donor's genotype. iPSCs represent therefore a valuable tool for regenerative medicine. Cardiac differentiation of ESCs can be enhanced via microRNAs (miRNAs) and small chemical compounds, which probably act as chromatin remodelers. Cardiomyogenic potential of iPSCs is currently intensely investigated for cell therapy or in vitro drug screening and disease modeling. However, influences of small compounds on iPSC-related cardiomyogenesis have not yet been investigated in details. Here, we compared the effects of two small molecules, bis-peroxo-vanadium (bpV) and sulfonyl-hydrazone-1 (SHZ) at varying concentrations, during cardiac differentiation of murine iPSCs. SHZ (5 µM) enhanced specific marker expression and cardiomyocyte yield, without loss of cell viability. In contrast, bpV showed negligible effects on cardiac differentiation rate and appeared to induce Casp3-dependent apoptosis in differentiating iPSCs. Furthermore, SHZ-treated iPSCs were able to increase beating foci rate and upregulate early and late cardiomyogenic miRNA expression (miR-1, miR-133a, and miR-208a). Thus, our results demonstrate that small chemical compounds, such as SHZ, can constitute a novel and clinically feasible strategy to improve iPSC-derived cardiac differentiation.
Proceedings of the National Academy of Sciences of the United States of America 2008 Cardiogenic small molecules that enhance myocardial repair by stem cells. Sadek H et al.

Abstract

The clinical success of stem cell therapy for myocardial repair hinges on a better understanding of cardiac fate mechanisms. We have identified small molecules involved in cardiac fate by screening a chemical library for activators of the signature gene Nkx2.5, using a luciferase knockin bacterial artificial chromosome (BAC) in mouse P19CL6 pluripotent stem cells. We describe a family of sulfonyl-hydrazone (Shz) small molecules that can trigger cardiac mRNA and protein expression in a variety of embryonic and adult stem/progenitor cells, including human mobilized peripheral blood mononuclear cells (M-PBMCs). Small-molecule-enhanced M-PBMCs engrafted into the rat heart in proximity to an experimental injury improved cardiac function better than control cells. Recovery of cardiac function correlated with persistence of viable human cells, expressing human-specific cardiac mRNAs and proteins. Shz small molecules are promising starting points for drugs to promote myocardial repair/regeneration by activating cardiac differentiation in M-PBMCs.
View All Publications

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