EasySep™ Non-Human Primate B Cell Isolation Kit

Immunomagnetic negative selection kit
Catalog #
100-0345_C
Immunomagnetic negative selection kit
From: 975 USD
Required Products
  1. EasySep™ Magnet
    EasySep™ Magnet

    Magnet for column-free immunomagnetic separation

  2. EasySep™ Buffer
    EasySep™ Buffer

    Cell separation buffer

  3. Anti-Human CD20 Antibody, Clone 2H7
    Anti-Human CD20 Antibody, Clone 2H7

    Mouse monoclonal IgG2b antibody against human, rhesus, cynomolgus CD20

Overview

The EasySep™ Non-Human Primate B Cell Isolation Kit is designed to isolate untouched and highly purified B cells from fresh or previously frozen non-human primate peripheral blood mononuclear cells (PBMCs) in as little as 20 minutes by immunomagnetic negative selection. The EasySep™ procedure involves labeling unwanted cells with antibody complexes and magnetic particles. The magnetically labeled cells are separated from the untouched desired cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube. Isolated cells are immediately available for downstream applications such as flow cytometry, culture, or DNA/RNA extraction.

This kit has been verified for use with rhesus and cynomolgus macaques.
Advantages
⦁ Fast, easy to use, and column-free
⦁ Up to 95% purity
⦁ Untouched, viable cells
Components
  • EasySep™ Non-Human Primate B Cell Isolation Kit (Catalog #100-0345)
    • EasySep™ Non-Human Primate B Cell Isolation Cocktail, 1 mL
    • EasySep™ Dextran RapidSpheres™, 2 x 1 mL
    • EasySep™ Isolation Cocktail Enhancer, 1 mL
  • RoboSep™ Non-Human Primate B Cell Isolation Kit (Catalog #100-0347)
    • EasySep™ Non-Human Primate B Cell Isolation Cocktail, 1 mL
    • EasySep™ Dextran RapidSpheres™, 2 x 1 mL
    • EasySep™ Isolation Cocktail Enhancer, 1 mL
    • RoboSep™ Buffer (Catalog #20104)
    • RoboSep™ Filter Tips (Catalog #20125)
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• RoboSep™-S (Catalog #21000)
Subtype
Cell Isolation Kits
Cell Type
B Cells
Species
Non-Human Primate
Sample Source
PBMC
Selection Method
Negative
Application
Cell Isolation
Brand
EasySep, RoboSep
Area of Interest
Immunology, Infectious Diseases

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
EasySep™ Non-Human Primate B Cell Isolation Kit
Catalog #
100-0345
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
RoboSep™ Non-Human Primate B Cell Isolation Kit
Catalog #
100-0347
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ Non-Human Primate B Cell Isolation Kit
Catalog #
100-0345
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ Non-Human Primate B Cell Isolation Kit
Catalog #
100-0345
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
EasySep™ Non-Human Primate B Cell Isolation Kit
Catalog #
100-0345
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
RoboSep™ Non-Human Primate B Cell Isolation Kit
Catalog #
100-0347
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
RoboSep™ Non-Human Primate B Cell Isolation Kit
Catalog #
100-0347
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
RoboSep™ Non-Human Primate B Cell Isolation Kit
Catalog #
100-0347
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
RoboSep™ Non-Human Primate B Cell Isolation Kit
Catalog #
100-0347
Lot #
All
Language
English

Educational Materials(4)

Brochure
EasySep™ Cell Separation Technology
Brochure
EasySep™: Isolate Cells from Non-Human Primates
Brochure
Tools For Your Immunology Research
Video
1:57
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

The content of rhesus macaque CD20+ B cells before and after isolation correspond to 10.4% and 94.8%, respectively.

Figure 1. EasySep™ Non-Human Primate B Cell Isolation Kit

Starting with rhesus PBMCs, the B cell content (CD20+) of the isolated fraction is typically 91.4 ± 5.2% (mean ± SD using the purple EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions are 10.4% and 94.8%, respectively.

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