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Data and Publications
(A) The biological activity of Mouse Recombinant IL-12 was tested by its ability to induce CD25 expression on mouse spleen CD8+ T
cells. Expression of CD25 on CD8+ T cells was measured after 72 hours of culture using a fluorometric assay method. The EC50 is
defined as the effective concentration of the growth factor at which expression of CD25 is at 50% of maximum. The EC50 in the above
example is 1 - 1.8 ng/mL.
(B) 1 ug of Mouse Recombinant IL-12 was resolved with SDS-Page under reducing (+) non-reducing (-) conditions and visualized by Coomassie Blue staining. Mouse Recombinant IL-12 is a heterodimer comprised of one p40 subunit and one p35 subunit. The total predicted molecular mass is 57.5 kDa but migrates to an apparent molecular mass of 70 kDa.