Mouse IL-12 (p70) ELISA Kit

For detection and measurement of mouse interleukin 12 p70 subunit

Mouse IL-12 (p70) ELISA Kit

For detection and measurement of mouse interleukin 12 p70 subunit

From: 750 USD
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For detection and measurement of mouse interleukin 12 p70 subunit
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What's Included

  • Mouse IL-12 (p70) ELISA Kit, 2 plates (Catalog #02026)
    • Mouse IL-12 (p70) ELISA Plate, 2x
    • Mouse IL-12 & IL-12/-23 Standard, 1 vial
    • Mouse IL-12 (p70) Biotinylated Detection Antibody, 30 µL
    • SA-HRP, 30 µL
    • Standard Reconstitution Buffer B, 1 mL
    • Wash Buffer (20X), 120 mL
    • ELISA Diluent, 120 mL
    • SA-HRP Diluent, 25 mL
    • TMB Substrate, 25 mL
    • Stop Solution, 25 mL
    • Adhesive Plate Covers, 6x
 
  • Mouse IL-12 (p70) ELISA Kit, 10 plates (Catalog #02027)
    • Mouse IL-12 (p70) ELISA Plate, 10x
    • Mouse IL-12 & IL-12/-23 Standard, 1 vial
    • Mouse IL-12 (p70) Biotinylated Detection Antibody, 130 µL
    • SA-HRP, 130 µL
    • Standard Reconstitution Buffer B, 1 mL
    • Wash Buffer (20X), 5 x 120 mL
    • ELISA Diluent, 3 x 120 mL
    • SA-HRP Diluent, 120 mL
    • TMB Substrate, 120 mL
    • Stop Solution, 120 mL
    • Adhesive Plate Covers, 30x

Overview

The Mouse Interleukin 12 (IL-12) p70 ELISA Kit is designed for the quantitative detection and measurement of mouse IL-12 (p70) in biological fluids such as serum, plasma, and cell culture supernatants. IL-12 (p70) is a heterodimeric cytokine composed of p35 and p40 subunits; the p40 subunit is shared with IL-23. IL-12 is produced by monocytes, macrophages, dendritic cells, neutrophils, and B cells. IL-12 is a regulator of cytokine synthesis, especially IFN-γ, enhances the proliferation of T and NK cells, and stimulates the differentiation of CD4+ and CD8+ T cells.

The assay is based on the sandwich ELISA method, in which samples are added to ELISA strip plates pre-coated with capture antibodies specific for the cytokine. The captured cytokine is detected by addition of a biotinylated detection antibody, followed by streptavidin-horseradish peroxidase, which binds the biotinylated antibody. Addition of the chromogenic enzyme substrate 3,3’,5,5’ tetramethylbenzidine (TMB) results in a colored product with an intensity directly proportional to the concentration of cytokine in the sample. The concentration of the cytokine is determined by comparison to a serial dilution of the cytokine standard analyzed in parallel.
Subtype
Complete Kits
Cell Type
B Cells, Dendritic Cells, Granulocytes and Subsets, Macrophages, Monocytes, T Cells
Species
Mouse
Application
ELISA
Area of Interest
Immunology

Data Figures

Representative Standard Curve

• Reportable range: 10 - 3160 pg/mL. This is the concentration range in which measurement of the analyte can be done with the highest precision, accuracy, and linearity.
• Sensitivity: The limit of detection of this assay is 6 pg/mL. This is the analyte concentration with absorbance two standard deviations higher than the zero standard.
• Accuracy: No international standard exists for calibration.
• Recovery: A mid-curve recovery of 85 - 121% was determined by spiking defined amounts of analyte standard into serum or plasma samples in repeated experiments.
• Precision: The intra-assay precision of this assay is 3.7% (CV). The inter-assay precision of this assay is 6.8% (CV).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

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02026, 02027
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English
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Safety Data Sheet 1
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02026, 02027
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English
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Safety Data Sheet 2
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02026, 02027
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English
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Safety Data Sheet 3
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02026, 02027
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English
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Safety Data Sheet 4
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02026, 02027
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English
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Safety Data Sheet 5
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02026, 02027
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English
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Safety Data Sheet 6
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02026, 02027
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Safety Data Sheet 7
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02026, 02027
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English
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Safety Data Sheet 8
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02026, 02027
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All
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English
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Safety Data Sheet 9
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02026, 02027
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All
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English
Catalog #
02026, 02027
Lot #
All
Language
English

Resources and Publications

Educational Materials (2)