Anti-Human CD71 (Transferrin Receptor) Antibody, Clone OKT9

Mouse monoclonal IgG1 antibody against human CD71 (transferrin receptor)

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Mouse monoclonal IgG1 antibody against human CD71 (transferrin receptor)
From: 119 USD

Overview Related Products Scientific Resources Product Applications Data and Publications

Overview

The OKT9 antibody reacts with CD71 (transferrin receptor), an ~180 - 190 kDa disulfide-bonded homodimer and type 2 transmembrane glycoprotein expressed at high levels on a broad range of actively proliferating cells, as well as reticulocytes, monocytes, macrophages and marrow stromal cells. Surface levels are low on resting leukocytes but are upregulated on lymphocytes, monocytes and macrophages following antigen or mitogen stimulation. Expression is lost during differentiation of reticulocytes into mature erythrocytes. CD71 mediates uptake of transferrin–iron complexes through receptor-mediated endocytosis and recycling of the apotransferrin–receptor complex to the cell surface. CD71 has been employed as a marker for evaluating erythroid precursors and for assessing disorders such as erythroid leukemia and myelodysplastic syndrome. It has also served as a target for drug delivery. The OKT9 antibody binds an epitope that reportedly overlaps with that of antibody clone CY1G4 but is distinct from the ligand binding site for transferrin.
Subtype:
Primary Antibodies
Target Antigen:
CD71 (Transferrin Receptor)
Alternative Names:
Mtvr1, p90, T9, TFR, TFRC, TFR1, TR, Transferrin receptor, TRFR
Reactive Species:
Human
Conjugation:
APC; Biotin; FITC; PE; Unconjugated
Host Species:
Mouse
Cell Type:
Erythroid Cells
Application:
Electron Microscopy; Flow Cytometry; Functional Assay; Immunofluorescence; Immunocytochemistry; Immunohistochemistry; Immunoprecipitation; Western Blotting
Area of Interest:
Stem Cell Biology
Clone:
OKT9
Gene ID:
7037
Isotype:
IgG1, kappa

Scientific Resources

Product Documentation

Educational Materials

(2)
Brochure
Hematopoietic Stem and Progenitor Cells - Products for Your Research
Brochure
Reliable Antibodies for Your Research

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area Workflow Stages for
Workflow Stages
B Cell Research
Cell Sourcing
Cell Isolation
Cell Expansion
Cell Characterization
Total Lymphocyte Research
Cell Sourcing
Cell Isolation
Cell Characterization

Data and Publications

Data

Data for Unconjugated

Figure 1. Data for Unconjugated

(A) Flow cytometry analysis of CD3+ cells (T cells) isolated from human peripheral blood mononuclear cells (PBMCs), then stimulated by incubation with anti-CD3 and anti-CD28 antibodies. Cells were labeled with Anti-Human CD71 (Transferrin Receptor) Antibody, Clone OKT9, followed by a rat anti-mouse IgG1 antibody, PE (filled histogram), or a mouse IgG1, kappa isotype control antibody followed by a rat anti-mouse IgG1 antibody, PE (solid line histogram).
(B) Flow cytometry analysis of CD3+ cells isolated from human PBMCs, then stimulated by incubation with anti-CD3 and anti-CD28 antibodies. Cells were labeled with Anti-Human CD71 (Transferrin Receptor) Antibody, Clone OKT9, which had been pre-incubated for 5 min with a rat anti-mouse IgG1 antibody, PE (filled histogram), or a mouse IgG1, kappa isotype control antibody, which had been pre-incubated for 5 min with a rat anti-mouse IgG1 antibody, PE (solid line histogram). Pre-incubating the primary and secondary antibodies helped minimize background binding of the secondary antibody to the anti-CD3 and anti-CD28 antibodies used for stimulating the cells.

Data for APC-Conjugated

Figure 2. Data for APC-Conjugated

Flow cytometry analysis of CD3+ cells (T cells) isolated from human peripheral blood mononuclear cells (PBMCs), then stimulated by incubation with anti-CD3 and anti-CD28 antibodies. Cells were labeled with Anti-Human CD71 (Transferrin Receptor) Antibody, Clone OKT9, APC (filled histogram) or a mouse IgG1, kappa isotype control antibody, APC (solid line histogram).

Data for Biotin-Conjugated

Figure 3. Data for Biotin-Conjugated

Flow cytometry analysis of CD3+ cells (T cells) isolated from human peripheral blood mononuclear cells (PBMCs), then stimulated by incubation with anti-CD3 and anti-CD28 antibodies. Cells were labeled with Anti-Human CD71 (Transferrin Receptor) Antibody, Clone OKT9, Biotin followed by streptavidin (SAV) APC (filled histogram), or a biotinylated mouse IgG1, kappa isotype control antibody followed by SAV APC (solid line histogram).

Data for FITC-Conjugated

Figure 4. Data for FITC-Conjugated

Flow cytometry analysis of CD3+ cells (T cells) isolated from human peripheral blood mononuclear cells (PBMCs), then stimulated by incubation with anti-CD3 and anti-CD28 antibodies. Cells were labeled with Anti-Human CD71 (Transferrin Receptor) Antibody, Clone OKT9, FITC (filled histogram) or a mouse IgG1, kappa isotype control antibody, FITC (solid line histogram).

Data for PE-Conjugated

Figure 5. Data for PE-Conjugated

Flow cytometry analysis of CD3+ cells (T cells) isolated from human peripheral blood mononuclear cells (PBMCs), then stimulated by incubation with anti-CD3 and anti-CD28 antibodies. Cells were labeled with Anti-Human CD71 (Transferrin Receptor) Antibody, Clone OKT9, PE (filled histogram) or a mouse IgG1, kappa isotype control antibody, PE (solid line histogram).

Alexa Fluor is a registered trademark of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor® are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.