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Anti-Human CD43 Antibody, Clone CD43-10G7

Mouse monoclonal IgG1 antibody against human CD43

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Anti-Human CD43 Antibody, Clone CD43-10G7

Mouse monoclonal IgG1 antibody against human CD43

From: 153 USD
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Mouse monoclonal IgG1 antibody against human CD43
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Overview

The CD43-10G7 antibody reacts with human CD43, a type I transmembrane sialoglycoprotein expressed on the surface of most human hematopoietic cells, including thymocytes, peripheral T cells, NK cells, monocytes, granulocytes, some B cells (activated B cells and an early B cell subset in bone marrow), plasma cells, and hematopoietic progenitor cells. Low expression levels are observed on platelets. CD43 exhibits different glycoforms in the range of ~95 - 135 kDa, depending on the cell type. It binds ligands such as CD54 and CD169 (Siglec-1) and plays regulatory roles in cell adhesion and activation, apoptosis and tumor progression, and is used a marker for discriminating normal from neoplastic T cells and B cells within tissues. Binding of the CD43-10G7 antibody to CD43 on neutrophils reportedly induces homotypic cell adhesion.
Subtype
Primary Antibodies
Target Antigen
CD43
Alternative Names
Leukocyte sialoglycoprotein, Leukosialin, Ly-48, Sialophorin, W3/13
Reactive Species
Human
Conjugation
APC, PE, Unconjugated
Host Species
Mouse
Cell Type
B Cells
Species
Human
Application
Flow Cytometry, Functional Assay, Immunoprecipitation
Area of Interest
Immunology
Clone
CD43-10G7
Gene ID
6693
Isotype
IgG1, kappa

Data Figures

Data for APC-Conjugated

Figure 1. Data for APC-Conjugated

(A) Flow cytometry analysis of human buffy coat nucleated cells labeled with Anti-Human CD43 Antibody, Clone CD43-10G7, APC and Anti-Human CD45 Antibody, Clone HI30, PE (Catalog #60018PE).
(B) Flow cytometry analysis of human buffy coat nucleated cells processed with the EasySep™ HLA Whole Blood B Cell Positive Selection Kit and labeled with Anti-Human CD43 Antibody, Clone CD43-10G7, APC and Anti-Human CD45 Antibody, Clone HI30, PE. Note: CD43 is not expressed on resting B cells.
(C) Flow cytometry analysis of human buffy coat nucleated cells labeled with a mouse IgG1, kappa APC isotype control antibody and Anti-Human CD45 Antibody, Clone HI30, PE.

Data for PE-Conjugated

Figure 2. Data for PE-Conjugated

(A) Flow cytometry analysis of human buffy coat nucleated cells labeled with Anti-Human CD43 Antibody, Clone CD43-10G7, PE and Anti-Human CD45 Antibody, Clone HI30, APC (Catalog #60018AZ).
(B) Flow cytometry analysis of human buffy coat nucleated cells processed with the EasySep™ HLA Whole Blood B Cell Positive Selection Kit and labeled with Anti-Human CD43 Antibody, Clone CD43-10G7, PE and Anti-Human CD45 Antibody, Clone HI30, APC. Note: CD43 is not expressed on resting B cells.
(C) Flow cytometry analysis of human buffy coat nucleated cells labeled with a mouse IgG1, kappa PE isotype control antibody and Anti-Human CD45 Antibody, Clone HI30, APC.

Data for Unconjugated

Figure 3. Data for Unconjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD43 Antibody, Clone CD43-10G7, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (Catalog #60138FI) (filled histogram), or a mouse IgG1, kappa isotype control antibody, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (solid line histogram).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
Anti-Human CD43 Antibody, Clone CD43-10G7, APC
Catalog #
60085AZ, 60085AZ.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD43 Antibody, Clone CD43-10G7, PE
Catalog #
60085PE.1, 60085PE
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD43 Antibody, Clone CD43-10G7
Catalog #
60085
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD43 Antibody, Clone CD43-10G7, APC
Catalog #
60085AZ, 60085AZ.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD43 Antibody, Clone CD43-10G7, PE
Catalog #
60085PE.1, 60085PE
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD43 Antibody, Clone CD43-10G7
Catalog #
60085
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (4)

Reliable Antibodies for Your Research
Brochure
Reliable Antibodies for Your Research
HLA Antibodies for Purity Assessment
Brochure
HLA Antibodies for Purity Assessment
Tools for Your Immunology Research
Brochure
Tools for Your Immunology Research
Human Immune Cytokines
Wallchart
Human Immune Cytokines

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Quality Statement:

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.

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