7-AAD (7-Aminoactinomycin D)

Cell viability dye (DNA-labeling dye)

7-AAD (7-Aminoactinomycin D)

Cell viability dye (DNA-labeling dye)

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Cell viability dye (DNA-labeling dye)
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Overview

7‐AAD (7‐Aminoactinomycin D) is a fluorescent cell viability dye which is excluded from live cells with intact membranes but penetrates dead or damaged cells and binds to double‐stranded DNA with high affinity by intercalating between GC base pairs. It is often used as an alternative to propidium iodide (PI; Catalog #75002) to differentiate and exclude non-viable cells in flow cytometric analyses, and can be excited using 488 nm (blue), 532 nm (green), and 561 nm (yellow-green) laser lines. Compared to PI, 7-AAD offers the advantage of having minimal overlap in its emission spectra with mostly blue and green, and many red fluorophores, including the commonly used phycoerythrin (PE) and fluorescein isothiocyanate (FITC). 7‐AAD is also used in DNA fluorescence imaging applications to discriminate early and late stages of apoptosis, to study cell‐mediated cytotoxicity, and for chromosome banding analysis. 7‐AAD has been found to exhibit antibacterial properties and exhibits growth‐inhibitory activity against certain types of leukemia and sarcoma.
Alternative Names
2,7-diamino-4,6-dimethyl-3-oxo-1-N,9-N-bis[7,11,14-trimethyl-2,5,9,12,15-pentaoxo-3,10-di(propan-2-yl)-8- oxa-1,4,11,14-tetrazabicyclo[14.3.0]nonadecan-6-yl]phenoxazine-1,9-dicarboxamide; 3H-Phenoxazine, actinomycin D deriv.; 7-Aminodactinomycin; Actinomy
Cell Type
Other
Species
Human, Mouse, Non-Human Primate, Other, Rat
Application
Flow Cytometry, Immunocytochemistry, Immunofluorescence
Area of Interest
Immunology, Neuroscience, Stem Cell Biology
CAS Number
7240-37-1
Chemical Formula
C₆₂H₈₇N₁₃O₁₆
Molecular Weight
1270.4 g/mol

Data Figures

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with 7-AAD (7-Aminoactinomycin D)

Figure 1. (A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with 7-AAD (7-Aminoactinomycin D)

(B) Chemical structure of 7-AAD (7-Aminoactinomycin D).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
75001, 75001.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
75001, 75001.1
Lot #
All
Language
English

Applications

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Resources and Publications

Educational Materials (1)