7-AAD (7-Aminoactinomycin D)

Cell viability dye (DNA-labeling dye)
Catalog #
75001_C
Cell viability dye (DNA-labeling dye)
From: 38 EUR

Overview

7‐AAD (7‐Aminoactinomycin D) is a fluorescent cell viability dye which is excluded from live cells with intact membranes but penetrates dead or damaged cells and binds to double‐stranded DNA with high affinity by intercalating between GC base pairs. It is often used as an alternative to propidium iodide (PI; Catalog #75002) to differentiate and exclude non-viable cells in flow cytometric analyses, and can be excited using 488 nm (blue), 532 nm (green), and 561 nm (yellow-green) laser lines. Compared to PI, 7-AAD offers the advantage of having minimal overlap in its emission spectra with mostly blue and green, and many red fluorophores, including the commonly used phycoerythrin (PE) and fluorescein isothiocyanate (FITC). 7‐AAD is also used in DNA fluorescence imaging applications to discriminate early and late stages of apoptosis, to study cell‐mediated cytotoxicity, and for chromosome banding analysis. 7‐AAD has been found to exhibit antibacterial properties and exhibits growth‐inhibitory activity against certain types of leukemia and sarcoma.
Cell Type
Other
Species
Human, Mouse, Rat, Non-Human Primate, Other
Application
Flow Cytometry, Immunocytochemistry, Immunofluorescence
Area of Interest
Immunology, Neuroscience, Stem Cell Biology
CAS Number
7240-37-1
Chemical Formula
C₆₂H₈₇N₁₃O₁₆
Molecular Weight
1270.4 g/mol

Related Products

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
7-AAD (7-Aminoactinomycin D)
Catalog #
75001, 75001.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
7-AAD (7-Aminoactinomycin D)
Catalog #
75001, 75001.1
Lot #
All
Language
English

Educational Materials(1)

Wallchart
Human Immune Cytokines

Data and Publications

Data

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with 7-AAD (7-Aminoactinomycin D)

Figure 1. (A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with 7-AAD (7-Aminoactinomycin D)

(B) Chemical structure of 7-AAD (7-Aminoactinomycin D).

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