cell-derived hematopoietic progenitor cells in defined serum-free conditions. MethoCult™ SF H4636 is formulated to support optimal growth of erythroid progenitor cells (CFU-E and BFU-E), granulocyte-macrophage progenitor cells (CFU-GM, CFU-G, CFU-M), and multipotential progenitor cells (CFU-GEMM; granulocyte, erythrocyte, macrophage, megakaryocyte).
MethoCult™ SF H4636 is also recommended for CFU assays with mononuclear cells, CD34+ enriched cells, and cells isolated by other purification methods from human bone marrow (BM), mobilized peripheral blood (MPB), peripheral blood (PB), and cord blood (CB) samples.
• Bovine serum albumin
• Recombinant human insulin
• Human transferrin (iron-saturated)
• Cytokines including recombinant human erythropoietin (EPO)
Frequently Asked Questions
Why use semi-solid media?
Why use methylcellulose-based media?
Is it necessary to add antibiotics to the media?
Is there anything I can do if my cultures appear contaminated?
Why can't I use a pipette to dispense methylcellulose-based media?
Can I 'pluck' the colonies for individual analysis?
Why are low adherence dishes so important?
Can MethoCult™ products be used for lymphoid progenitor CFU assays?
Is it possible to set up CFU assays in a 24-well plate?
Can I stain colonies in MethoCult™ medium?
Are there differences in colony morphology with serum-free media?
Can MethoCult™ be made with alternate base media?
Is there a MethoCult™ formulation suitable for HPP-CFC (high proliferative potential colony forming cell)?
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Data and Publications
Figure 1. Procedure Summary for Hematopoietic CFU Assays
Figure 2. Total Colony Numbers of CD34+ Cells Grown in MethoCult™ SF H4436 and MethoCult™ SF H4636
CD34+ and mononuclear cells derived from CB, mPB and BM were isolated and plated in MethoCult™ media and counted after 14 days. Shown are the number of total colonies grown in serum-free MethoCult™ SF H4436 (Catalog #04436) and MethoCult™ SF H4636 (Catalog #04636) normalized relative to total colonies grown in serum-containing MethoCult™ H4435 (Catalog #04435). MethoCult™ SF H4636 provides improved conditions for colony growth in serum-free conditions as compared to MethoCult™ SF H4436. Shown are means with standard deviation (n = 6).