EasySep™ Direct HLA Crossmatch B Cell Isolation Kit

Immunomagnetic negative selection from whole blood, spleen, or lymph nodes kit
Catalog #
19684_C
Immunomagnetic negative selection from whole blood, spleen, or lymph nodes kit
From: 476 USD
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Required Products
  1. EasySep™ Magnet
    EasySep™ Magnet

    Magnet for column-free immunomagnetic separation

  2. D-PBS Without Ca++ and Mg++
    D-PBS (Without Ca++ and Mg++)

    Dulbecco’s phosphate-buffered saline without calcium and magnesium

Overview

The EasySep™ Direct HLA Crossmatch B Cell Isolation Kit can be used to isolate highly purified B cells directly from human whole blood, spleen, or lymph nodes by immunomagnetic negative selection. This cell isolation kit targets non-B cells for removal with antibodies recognizing specific surface markers. Unwanted cells are labeled with antibodies and EasySep™ Direct RapidSpheres™, and separated using an EasySep™ magnet. Desired cells are simply collected into a new tube and and are immediately available for downstream applications such as flow cytometry crossmatch.
Advantages
• > 99.9% RBC depletion without the need for density gradient centrifugation, sedimentation, or lysis
• Up to 99.8% purity of isolated cells
• Fast, easy-to-use, and column-free
• Isolated cells are untouched
Components
  • EasySep™ Direct HLA Crossmatch B Cell Isolation Kit (Catalog #19684)
    • EasySep™ Direct HLA Crossmatch B Cell Isolation Cocktail, 2 x 2.5 mL
    • EasySep™ Direct RapidSpheres™, 4 x 2.5 mL
  • EasySep™ Direct HLA Crossmatch B Cell Isolation Kit for RoboSep™ (Catalog #19684RF)
    • EasySep™ Direct HLA Crossmatch B Cell Isolation Cocktail, 2 x 2.5mL
    • EasySep™ Direct RapidSpheres™, 4 x 2.5 mL
    • RoboSep™ Buffer (Catalog #20104)
    • RoboSep™ Filter Tips (Catalog #20125)
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• Easy 50 EasySep™ Magnet (Catalog #18002)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• RoboSep™-S (Catalog #21000)
Subtype
Cell Isolation Kits
Cell Type
B Cells
Species
Human
Sample Source
Lymph Node, Spleen, Whole Blood
Selection Method
Negative
Application
Cell Isolation
Brand
EasySep
Area of Interest
HLA, Immunology

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
EasySep™ Direct HLA Crossmatch B Cell Isolation Kit
Catalog #
19684
Lot #
18F92330 or higher
Language
English
Document Type
Product Information Sheet
Product Name
EasySep™ Direct HLA Crossmatch B Cell Isolation Kit for RoboSep™
Catalog #
19684RF
Lot #
18F92330 or higher
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ Direct HLA Crossmatch B Cell Isolation Kit
Catalog #
19684
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ Direct HLA Crossmatch B Cell Isolation Kit
Catalog #
19684
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ Direct HLA Crossmatch B Cell Isolation Kit for RoboSep™
Catalog #
19684RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ Direct HLA Crossmatch B Cell Isolation Kit for RoboSep™
Catalog #
19684RF
Lot #
All
Language
English

Educational Materials (11)

Brochure
Human B Cell Isolation Product Selection
Brochure
HLA Antibodies for Purity Assessment
Brochure
Cell Separation Products for HLA Analysis
Brochure
EasySep™ Cell Separation Technology
Technical Bulletin
Cell Separation Solutions for the Flow Cytometric Crossmatch Assay
Wallchart
Frequencies of Cell Types in Human Peripheral Blood
Wallchart
Human Immune Cytokines
Video
Cell Isolation Directly from Whole Blood without RBC Lysis or Centrifugation: EasySep™ Direct
1:44
Cell Isolation Directly from Whole Blood without RBC Lysis or Centrifugation: EasySep™ Direct
Video
Automate Cell Isolation with the RoboSep™-S Cell Separation Instrument
1:41
Automate Cell Isolation with the RoboSep™-S Cell Separation Instrument
Scientific Poster
Immunomagnetic Cell Isolation of Untouched Lymphocyte Populations Directly from Whole Blood
Scientific Poster
Rapid, Automated Lymphocyte Isolation Directly from Whole Blood with EasySep™ Direct

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Data and Publications

Data

Starting with human whole blood from normal healthy donors, the B cell (CD19+) content of the non-lysed final isolated fraction typically ranges from 89 - 99.8% (gated on CD45). In the above example, the B cell (CD19+) content of the lysed whole blood start sample and non-lysed final isolated fraction is 12.3% and 98.4% (gated on CD45), respectively.

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