Mouse Recombinant IL-12
Interleukin 12
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Mouse Recombinant IL-12 -
Label for Mouse Recombinant IL-12 -
Label for Mouse Recombinant IL-12
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Overview
Interleukin 12 (IL-12p70) is a heterodimeric cytokine composed of p35 and p40 subunits. IL-12 is produced by monocytes, macrophages, dendritic cells, neutrophils and B cells in response to bacterial products and cytokines such as IFN-γ. The IL-12 receptor is expressed on T, NK, and dendritic cells. Upon binding, IL-12 initiates signaling via the JAK/STAT signaling pathway and stimulates NK, B, and T cells to produce IFN-γ (Watford et al.). It also regulates cytokine synthesis, proliferation of T and NK cells, and stimulates differentiation of CD4+ and CD8+ T cells (Germann & Rüde). Mice that are deficient in IL-12 are susceptible to many intracellular pathogens and have impaired IFN-γ secretion, Th1 differentiation and NK cytolytic activity; however, Th2 development and IL-4 production are enhanced (Watford et al.).
Alternative Names:
CLMF, CTL maturation factor, Cytotoxic lymphocyte maturation factor, IL-12p70, Interleukin-12, Natural killer cells stimulator factor, NKSF
Cell Type:
B Cells; Hematopoietic Stem and Progenitor Cells; Lymphocytes; NK Cells; T Cells
Species:
Mouse
Area of Interest:
Immunology; Stem Cell Biology
Molecular Weight:
57.5 kDa
Purity:
≥ 95%
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Data
(A) The biological activity of Mouse Recombinant IL-12 was tested by its ability to induce CD25 expression on mouse spleen CD8+ T
cells. Expression of CD25 on CD8+ T cells was measured after 72 hours of culture using a fluorometric assay method. The EC50 is
defined as the effective concentration of the growth factor at which expression of CD25 is at 50% of maximum. The EC50 in the above
example is 1 - 1.8 ng/mL.
(B) 1 ug of Mouse Recombinant IL-12 was resolved with SDS-Page under reducing (+) non-reducing (-) conditions and visualized by
Coomassie Blue staining. Mouse Recombinant IL-12 is a heterodimer comprised of one p40 subunit and one p35 subunit. The total
predicted molecular mass is 57.5 kDa but migrates to an apparent molecular mass of 70 kDa.
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.