Mouse Recombinant IFN beta

Interferon beta

Mouse Recombinant IFN beta

Interferon beta

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Interferon beta
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Overview

A cytokine belonging to the type 1 interferon family, Interferon beta (IFN beta) binds IFN alpha/beta receptors (IFNAR) that activate tyrosine kinases and initiate the interferon-induced Jak-STAT signaling pathway, which modulates many key immune processes (Smieja et al.). In an experimental model involving cardiac fibroblasts isolated from rats, IFN beta was found to induce both pro- and anti-inflammatory cytokines production by activating different STAT proteins (Bolivar et al.). The anti-inflammatory effects of IFN beta have been studied in the context of autoimmune disorders, and there are currently multiple approved IFN beta drugs for treatment of relapsing forms of multiple sclerosis (Filipi and Jack). IFN beta is produced by immune cells, including macrophages, and non-immune cells, such as fibroblasts and epithelial cells (Ivashkiv and Donalin). The crystal structure of IFN beta shares characteristics with other type I interferons. It comprises five alpha-helices with four of them forming a helix bundle, and one long and three shorter loops connecting the helices (Karpusas et al.). For consistency and reproducibility across your applications, interferon alpha 1 from STEMCELL comes lyophilized with ≥ 87% purity, specific activity EC50 ≤ 18 pg/mL, and LAL analysis verification ensuring endotoxin levels are ≤ 1.0 EU/μg protein.
Subtype
Cytokines
Alternative Names
IFB, IFNB
Species
Mouse
Area of Interest
Immunology
Purity
≥ 87%

Data Figures

(A) The biological activity of Mouse Recombinant IFN beta was tested by its ability to promote the viability of L929 cells infected with VSV in a cytopathic effect (CPE) assay. Cell viability was measured using a fluorometric assay method. The EC50 is defined as the effective concentration of the cytokine at which cell survival is at 50% of maximum. The EC50 in the above example is ≤18 pg/mL. (B) Mouse Recombinant IFN beta was resolved with SDS-PAGE under reducing (+) conditions and visualized by Coomassie Blue staining. Mouse Recombinant IFN beta has a predicted molecular mass of 20.3 kDa and an apparent molecular mass of 30 kDa due to glycosylation.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
100-1337
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
100-1337
Lot #
All
Language
English