Mouse ACE2 ELISA Kit

For detection and measurement of mouse ACE2
Mouse ACE2 ELISA Kit

For detection and measurement of mouse ACE2

1 Kit
Catalog # 100-0688
725 USD

Overview

The Mouse ACE2 ELISA Kit is designed for the quantitative detection and measurement of mouse angiotensin converting enzyme 2 (ACE2) in biological fluids such as serum, plasma, cell culture supernatant, and tissue homogenate.

ACE2 is a type I integral membrane protein. It is highly expressed in capillary-rich organs such as the lungs, heart, blood vessels, kidneys, liver, and gastrointestinal tract. The ACE2 receptor is the target of the SARS-CoV-2 Spike (S) glycoprotein. This interaction allows for viral entry into the host cell, making ACE2 a potential target for antiviral therapeutics.

The assay is based on the sandwich ELISA method, in which samples are added to ELISA strip plates pre-coated with capture antibodies specific for ACE2. The captured ACE2 is detected by addition of a biotinylated detection antibody, followed by streptavidin-horseradish peroxidase, which binds the biotinylated antibody. Addition of the chromogenic enzyme substrate 3,3’,5,5’ tetramethylbenzidine (TMB) results in a colored product with an intensity directly proportional to the concentration of ACE2 in the sample. The concentration of ACE2 is determined by comparison to a serial dilution of the standard analyzed in parallel.
Components
  • Mouse ACE2 ELISA Kit(Catalog #100-0XXX)
    • Mouse ACE2 ELISA Plate, 1x
    • Mouse ACE2 Standard, 2 vials
    • Mouse ACE2 Biotinylated Detection Antibody, 120 µL
    • SA-HRP, 120 µL
    • Mouse ACE2 Antibody Diluent, 15 mL
    • SA-HRP Diluent, 15 mL
    • ELISA Diluent, 50 mL
    • Wash Buffer (25X), 20 mL
    • TMB Substrate, 10 mL
    • Stop Solution, 10 mL
    • Adhesive Plate Covers, 4x
Subtype
Complete Kits
Reactive Species
Mouse
Application
ELISA
Area of Interest
Immunology, Infectious Diseases
Target
ACE2

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
Mouse ACE2 ELISA Kit
Catalog #
100-0688
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
Mouse ACE2 ELISA Kit
Catalog #
100-0688
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
Mouse ACE2 ELISA Kit
Catalog #
100-0688
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
Mouse ACE2 ELISA Kit
Catalog #
100-0688
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
Mouse ACE2 ELISA Kit
Catalog #
100-0688
Lot #
All
Language
English
Document Type
Safety Data Sheet 5
Product Name
Mouse ACE2 ELISA Kit
Catalog #
100-0688
Lot #
All
Language
English
Document Type
Safety Data Sheet 6
Product Name
Mouse ACE2 ELISA Kit
Catalog #
100-0688
Lot #
All
Language
English
Document Type
Safety Data Sheet 7
Product Name
Mouse ACE2 ELISA Kit
Catalog #
100-0688
Lot #
All
Language
English
Document Type
Safety Data Sheet 8
Product Name
Mouse ACE2 ELISA Kit
Catalog #
100-0688
Lot #
All
Language
English
Document Type
Safety Data Sheet 9
Product Name
Mouse ACE2 ELISA Kit
Catalog #
100-0688
Lot #
All
Language
English
Document Type
Safety Data Sheet 10
Product Name
Mouse ACE2 ELISA Kit
Catalog #
100-0688
Lot #
All
Language
English

Educational Materials (5)

Brochure
Tools For Your Immunology Research
Brochure
ELISA Kits for Immunology Research
Wallchart
SnapShot: COVID-19
Video
Collaborating to Accelerate COVID-19 Research
3:36
Collaborating to Accelerate COVID-19 Research
Webinar
SARS-CoV-2 Viral Diversity: Causes, Consequences, and Opportunities
45:33
SARS-CoV-2 Viral Diversity: Causes, Consequences, and Opportunities

Data and Publications

Data

Representative standard curve for Mouse ACE2 ELISA Kit

Figure 1. Representative Standard Curve

The reportable range (the concentration range in which measurement of the analyte can be done with the highest precision, accuracy, and linearity) of this assay is 6.25 - 400 pg/mL. The lower limit of detection (LLD) of this assay is 1.56 pg/mL. This is the lowest protein concentration that could be differentiated from zero as determined by the mean OD value of 20 replicates of the zero standard added by their three standard deviations. The intra- and inter-assay precision (CV) of this assay are < 8% and < 10%, respectively. This assay has high specificity for the detection of mouse ACE2. No significant cross-reactivity or interference is known to occur between mouse ACE2 and analogues.

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