MesenCult™-hPL Medium Kit

For the culture of human mesenchymal stem cells
MesenCult™-hPL Medium Kit

For the culture of human mesenchymal stem cells

1 Kit
Catalog # 05439
399 USD

Overview

MesenCult™-hPL Medium has been optimized for the culture and expansion of human mesenchymal stromal cells (MSCs; also known as mesencyhymal stem cells) as well as for the detection and enumeration of colony-forming unit fibroblasts (CFU-F). MesenCult™-hPL 10X Supplement is an alternative growth supplement to fetal bovine serum (FBS) that contains purified human platelet lysate (hPL). Complete MesenCult™-hPL Medium (Basal Medium + 10X Supplement) does not require addition of growth factors, lipids, or attachment substrate. It is more defined and provides better performance than FBS-containing media, and is also a more affordable option than defined, serum-free media.

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Advantages
• Superior performance in terms of CFU-F numbers and long-term expansion compared to FBS-containing medium
• Cheaper medium compared to serum-free, defined media
• More consistent and sustainable raw materials (less batch-to-batch variability)
• Complete supplement; addition of growth factor is unnecessary
• Attachment substrate is not required; saves time and money
Components
  • MesenCult™-hPL Basal Medium, 450 mL
  • MesenCult™-hPL 10X Supplement, 50 mL
Subtype
Specialized Media
Cell Type
Mesenchymal Stem and Progenitor Cells
Species
Human
Application
Cell Culture, Colony Assay, Expansion
Brand
MesenCult
Area of Interest
Stem Cell Biology

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
MesenCult™-hPL Medium Kit
Catalog #
05439
Lot #
Supplement 17E78846 or higher
Language
English
Document Type
Safety Data Sheet 1
Product Name
MesenCult™-hPL Medium Kit
Catalog #
05439
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
MesenCult™-hPL Medium Kit
Catalog #
05439
Lot #
All
Language
English

Educational Materials (7)

Brochure
qPCR Arrays for Cell Characterization
Brochure
Products for Your Mesenchymal Stromal Cell Research
Brochure
MesenCult™-hPL Medium For the Derivation and Expansion of Human Mesenchymal Stem and Progenitor Cells
Video
MesenCult™ for Mesenchymal Stromal Cell Derivation, Culture & Differentiation
1:51
MesenCult™ for Mesenchymal Stromal Cell Derivation, Culture & Differentiation
Video
Isolation of Umbilical Cord Mesenchymal Stromal Cells Using Explant Method
13:45
Isolation of Umbilical Cord Mesenchymal Stromal Cells Using Explant Method
Webinar
The Impact of Ancillary Materials on Your Translational Research
17:18
The Impact of Ancillary Materials on Your Translational Research
Webinar
The Secret In Vivo Life of "Mesenchymal Stem Cells"
58:00
The Secret In Vivo Life of "Mesenchymal Stem Cells"

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Figure 1. Bone marrow (BM)-derived MSCs Cultured in MesenCult™-hPL Medium Expand Faster than MSCs Cultured in Serum (FBS)-containing medium

Figure 2. Human BM-derived MSCs Cultured in MesenCult™-hPL Medium Display Multi-Lineage Differentiation Potential

(A) Human BM-derived MSCs cultured in MesenCult™-HPL Medium differentiate into (B) adipocytes (Oil Red O staining); and (C) chondrocytes (Alcian Blue staining); and (D) osteoblasts (alkaline phosphatase and von Kossa staining).

Figure 3. Flow Cytometric Analysis of Cultured-Expanded MSCs in MesenCult™-hPL Medium

BM-derived MSCs were cultured and expanded in MesenCult™-hPL Medium. MSCs were stained for the mesenchymal surface markers CD73, CD90 and CD105, and the hematopoietic marker, CD45. MSCs express high levels of CD73, CD90 and CD105 while lacking expression of CD45.

Publications (1)

Cellular {\&} molecular immunology 2020 oct Improved therapeutic consistency and efficacy of mesenchymal stem cells expanded with chemically defined medium for systemic lupus erythematosus. J. Xu et al.

Abstract

View All Publications

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