MesenCult™ Adipogenic Differentiation Kit (Mouse)

For the in vitro differentiation of mouse MSCs, ADSCs, and MEFs into adipocytes

More Views

MesenCult™ Adipogenic Differentiation Kit (Mouse)

For the in vitro differentiation of mouse MSCs, ADSCs, and MEFs into adipocytes

1 Kit
Catalog #05507
227 USD

Required Products

Overview

MesenCult™ Adipogenic Differentiation Kit (Mouse) is specifically formulated for the in vitro differentiation of mouse mesenchymal stem and progenitor cells (MSCs), adipose tissue-derived MSCs (ADSCs), and mouse embryonic fibroblasts (MEFs) into cells of the adipogenic lineage.
NOTE: MesenCult™ Adipogenic Differentiation Medium must be supplemented with L-Glutamine (Catalog #07100).
Advantages:
• Compatible with mouse MSCs previously culture-expanded using the MesenCult™ Expansion Kit (Mouse).
• Available in an easy-to-use two-component format.
• Rigorous raw material screening and quality control minimize lot-to-lot variablity.
Components:
  • MesenCult™ MSC Basal Medium (Mouse), 200 mL
  • MesenCult™ Adipogenic Differentiation 10X Supplement (Mouse), 22 mL
Subtype:
Specialized Media
Cell Type:
Mesenchymal Stem and Progenitor Cells
Species:
Mouse
Application:
Differentiation
Brand:
MesenCult
Area of Interest:
Stem Cell Biology

Scientific Resources

Educational Materials

(3)

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Publications

(1)
Journal of biomedical materials research. Part A 2019 jul

Aligned fibrous decellularized cell derived matrices for mesenchymal stem cell amplification.

M. Ventre et al.

Abstract

Biochemical and biophysical stimuli of stem cell niches finely regulate the self-renewal/differentiation equilibrium. Replicating this in vitro is technically challenging, making the control of stem cell functions difficult. Cell derived matrices capture certain aspect of niches that influence fate decisions. Here, aligned fibrous matrices synthesized by MC3T3 cells were produced and the role of matrix orientation and stiffness on the maintenance of stem cell characteristics and adipo- or osteo-genic differentiation of murine mesenchymal stem cells (mMSCs) was investigated. Decellularized matrices promoted mMSC proliferation. Fibrillar alignment and matrix stiffness work in concert in defining cell fate. Soft matrices preserve stemness, whereas stiff ones, in presence of biochemical supplements, promptly induce differentiation. Matrix alignment impacts the homogeneity of the cell population, that is, soft aligned matrices ameliorate the spontaneous adipogenic differentiation, whereas stiff aligned matrices reduce cross-differentiation. We infer that mechanical signaling is a dominant factor in mMSC fate decision and the matrix alignment contributes to produce a more homogeneous environment, which results in a uniform response of cells to biophysical environment. Matrix thus produced can be obtained in vitro in a facile and consistent manner and can be used for homogeneous stem cell amplification or for mechanotransduction-related studies.
STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.