ISSCR 2017 - Accelerate Your Research

Visit Us at Booth #527

We would love to hear about your research! You can also talk to our scientists and learn about new products for Genome Editing, Cardiomyocyte Differentiation and Organoid Culture applications.
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>> Visit the ISSCR 2017 Annual Meeting program page

09:00 COREdinates FOCUS SESSION: Novel Solutions for iPS Cell Core Facilities

Organized by: COREdinates
Speaker: Andrew Gaffney, Senior Product Marketing Manager
Location: Level 2, Room 253ABC
Time: 09:00 - 12:00

18:30 POSTER: STEMdiff™ Hematopoietic Kit Reproducibly Generates Functional Hematopoietic Progenitor Cells from Human Pluripotent Stem Cells

Presented by: Melanie Kardel, Senior Scientist
Poster Board: #W-2051
Time: 18:30 - 19:30

18:30 POSTER: Simple Label-Free Monitoring of Robust Human Pluripotent Stem Cell Differentiation to Cardiomyocytes with the Maestro MEA System and STEMdiff™ Cardiomyocyte Products

Presented by: Vincenzo Macri, Senior Scientist
Poster Board: #W-3057
Time: 18:30 - 19:30

19:30 POSTER: Efficient Production and Processing of Cardiomyocytes from Human Pluripotent Stem Cells Using STEMdiff™ Cardiomyocyte Products

Presented by: Vincenzo Macri, Senior Scientist
Poster Board: #W-1034
Time: 19:30 - 20:30

19:30 POSTER: Long-Term Expansion of Mouse Hepatic Stem Cells in 3D Culture Using HepatiCult™, a Serum-Free Hepatic Organoid Expansion Medium

Presented by: Charis Segeritz-Walko, Scientist
Poster Board: #W-1186
Time: 19:30 - 20:30

19:30 POSTER: Efficient Establishment and Long-Term Maintenance of 3-Dimensional Human Small Intestinal and Colonic Organoids Using a Novel IntestiCult™ Organoid Growth Medium (Human)

Presented by: Ryan Conder, Senior Scientist
Poster Board: #W-2162
Time: 19:30 - 20:30

19:30 POSTER: Suspension Cultures of Human Pluripotent Stem Cells as Aggregates in Orbital Shake Flasks

Presented by: Eric Jervis, Principal Scientist
Poster Board: #W-2168
Time: 19:30 - 20:30

11:30 INNOVATION SHOWCASE: Intestinal and Cerebral Organoids: New Tools to Study Human Development and Diseases

Speakers:  Jordi Guiu Sagarra, Postdoctoral Fellow, Biotech Research & Innovation Center (BRIC)
 Oliver Wüseke, Postdoctoral Fellow, Institute of Molecular Biotechnology (IMBA)
Location: Level 2, Room 253ABC
Time: 11:30 - 12:30
*Lunch provided

18:00 POSTER: Increased Single Cell Cloning Efficiency of Human Pluripotent Stem Cells Using a Novel Cloning Supplement and Matrix

Presented by: Adam Hirst, Scientist
Poster Board: #T-2013
Time: 18:00 - 19:00

18:00 POSTER: NaïveCult Media System for the Generation and Maintenance of Chemically-Induced Reset Naïve Human Pluripotent Stem Cells

Presented by: Arwen Hunter, Senior Scientist
Poster Board: #T-2015
Time: 18:00 - 19:00

11:30 INNOVATION SHOWCASE: Highly Efficient Single-Cell Human Pluripotent Stem Cell Cloning and Robust Cardiomyocyte Differentiation

Speakers:  Adam Hirst, Scientist
 Vincenzo Macri, Senior Scientist
Location: Level 2, Room 253ABC
Time: 11:30 - 12:30
*Lunch provided

19:00 POSTER: STEMdiff™ Cerebral Organoid Kit: a New Tool for the Culture of 3-D Brain Organoids Derived from Human Pluripotent Stem Cells

Presented by: Vivian Lee, Associate Director, Neural & Pluripotent Stem Cell
Poster Board: #F-1192
Time: 19:00 - 20:00

Focus Session

Novel Solutions for iPS Cell Core Facilities

Wednesday, June 14, 09:00am - 12:00pm | Level 2, Room 253ABC

Organized by Stem Cell COREdinates.

Senior Product Marketing Manager, STEMCELL Technologies

Andrew Gaffney completed his PhD in Pediatric Oncology from the University of Leeds, UK, where he successfully established a human embryonic stem cell disease model of Ewing sarcoma. He joined STEMCELL Technologies in 2014 as a Global Technical Specialist where he provided advanced customer support in the areas of human pluripotent stem cell (hPSC) maintenance and differentiation. Since 2016, Andrew has been working as the Senior Product Marketing Manager for STEMCELL's PSC portfolio of products. He works closely with renowned stem cell scientists in both industry and academia to monitor the requirements needed for establishing successful hPSC-based disease models, drug screens and cell therapy applications.

Innovation Showcase 1

Intestinal and Cerebral Organoids: New Tools to Study Human Development and Diseases

Thursday, June 15, 11:30am - 12:30pm | Level 2, Room 253ABC

Cell culture models for studying human development have previously been limited to 2-D monolayer cultures derived from primary tissues or human pluripotent stem cells (hPSCs). Recently, the development of 3-D organoid models has enabled significant advances in developmental biology, disease modeling and patient-specific drug screening. Organoids contain progenitor cells and differentiated cell types, and can self-organize into 3-D structures that resemble their in vivo counterparts. Here, we present two of these models: intestinal and cerebral organoids.

Adult intestinal epithelial stem cells are located at the bottom of crypts of Lieberkühn. Using a combination of human and mouse intestinal organoids and in vivo fate mapping studies, we describe when, where and how cell fate, and stem cell identity are first established during development. Cerebral organoids derived from hPSCs can generate cell types and cell layers that recapitulate the complex organization of the human cortex. The derived brain tissues model early human fetal brain development and have been used to study diverse brain disorders such as microcephaly, schizophrenia and Zika virus infection.

Dr. Jordi Guiu Sagarra
Postdoctoral Fellow, the Jensen Lab
Biotech Research & Innovation Center (BRIC), University of Copenhagen

Jordi Guiu Sagarra obtained his PhD from the Anna Bigas Laboratory (Barcelona) where he studied the role of the Notch pathway in hematopoietic development. Later on he moved to the Kim Jensen Laboratory (Copenhagen). The main goal of his current project is to study Intestinal Stem Cell specification during development. Jordi Guiu is a recipient of a Marie Skłodowska-Curie Fellowship.

Dr. Oliver Wüseke
Postdoctoral Fellow, the Knoblich Lab
Institute of Molecular Biotechnology (IMBA)

A biochemist by training, Oliver Wüseke is a PostDoc in the Knoblich Lab at IMBA Vienna, who uses forebrain organoids to study the early development of the human brain. On top of this, Oliver has initiated a project that aims to establish a toolkit for present and future cerebral organoid researchers that will allow them to tailor the technology to their specific biological question. As a proud Wikipedia Donator, Oliver strongly believes in the value of free access to information and technology for the future of science and society.

Innovation Showcase 2

Highly Efficient Single-Cell Human Pluripotent Stem Cell Cloning and Robust Cardiomyocyte Differentiation

Friday, June 16, 11:30am - 12:30pm | Level 2, Room 253ABC

Recent advances in gene-editing techniques have led to more accessible and cost-effective methods to produce edited human pluripotent stem cell (hPSC) lines. However, low single-cell cloning efficiency (typically < 1%) remains a major limitation of this technology. To address this challenge, we developed a novel hPSC cloning supplement, CloneR™, that yields single-cell cloning efficiencies of 20-40% in mTeSR™1 and TeSR™-E8™ across multiple matrices. Gene-edited hPSC clones can be differentiated to specific cell types for disease modeling, drug discovery, or toxicology screening purposes.

The production and processing of hPSC-derived cardiomyocytes (hPSC-CMs) is both variable and cumbersome. To overcome this, we have developed an optimized workflow for hPSC-CM-based research, including cardiomyocyte differentiation, maintenance, dissociation, freezing, and support reagents. These STEMdiff™ Cardiomyocyte products facilitate reproducible and robust production and simple processing to yield high-quality hPSC-CMs (>80% cTnT + and >1x106 hPSC-CMs/well of a 12-well plate). In summary, this tutorial will highlight hPSC gene-editing and cardiac differentiation workflows using the CloneR™ supplement and the STEMdiff™ Cardiomyocyte product line.

Dr. Adam Hirst
Scientist
STEMCELL Technologies

Adam Hirst completed his PhD in Stem Cell Biology from the University of Sheffield where his research focused on genomic instability in human pluripotent stem cells. He then worked as a Research Fellow at the University of Nottingham studying the specification of primordial germ cells in non-rodent mammals. Adam is currently a Scientist at STEMCELL Technologies, developing products to facilitate innovative techniques such as gene-editing in human pluripotent stem cells.

Dr. Vincenzo Macri
Senior Scientist
STEMCELL Technologies

Vincenzo Macri completed his PhD in Physiology from the University of British Columbia. He then worked as a Postdoctoral Research Fellow at Massachusetts General Hospital and Harvard Medical School. His research work focused on human genetics, cardiac arrhythmias, cellular electrophysiology, and cardiomyocyte stem cell biology, for which he received Postdoctoral Fellowship awards from the Heart Rhythm Society and Massachusetts General Hospital. Vincenzo is currently a Senior Scientist at STEMCELL Technologies, developing products to support cardiomyocyte stem cell biology research.

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