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Data and Publications
(A) The biological activity of Human Recombinant RANKL, ACF was tested by its ability to induce production of embryonic alkaline phosphatase in RAW-Blue™ macrophage reporter cells. Alkaline phosphatase production was measured using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which alkaline phosphatase activity is at 50% of maximum. The EC50 in the above example is 38.8 ng/mL. (B) Human Recombinant RANKL, ACF was resolved with SDS-PAGE under reducing (+) conditions and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant RANKL, ACF has a predicted molecular mass of 19.7 kDa.