Data and Publications
(A) The biological activity of Human Recombinant IL-33 was tested by its ability to promote the proliferation of D10S cells in low serum
medium containing no other cytokines. Cell proliferation was measured after 67 hours of culture using a fluorometric assay method. The
EC50 is defined as the effective concentration of the growth factor at which cell proliferation is at 50% of maximum. The EC50 in the
above example is 0.1 - 0.15 ng/mL.
(B) 1 μg of Human Recombinant IL-33 was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant IL-33 has a predicted molecular mass of 18.1 kDa.