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Data and Publications
(A) The biological activity of Human Recombinant IL-18 BPa was tested by its ability to inhibit proliferation of KG-1 cells in the presence of 50 ng/mL human IL-18. Inhibition of cell proliferation was measured using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which cell proliferation inhibition is at 50% of maximum. The EC50 in the above example is 20 ng/mL. (B) Human Recombinant IL-18 BPa was resolved with SDS-PAGE under reducing (+) conditions and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant IL-18 BPa has a predicted molecular mass of 17.6 kDa (monomer)or 35.2 kDa (dimer).