Pluripotent Stem Cell Training in Wurzburg, Germany: May 26-28, 2020
Pluripotent stem cell (PSC) research is an expanding field that has the potential to change the way human diseases are studied and treated. With our three-day course, you can learn the techniques and protocols to help get your PSC research started. Guided by experts from STEMCELL and the Fraunhofer Institute, you will gain skills to successfully derive and maintain high-quality human induced pluripotent stem (iPS) cells from somatic cells and differentiate them towards specialized cell types. In this immersive learning experience, you will have the opportunity to experience adaptive practical sessions and engage with PSC experts from the Fraunhofer Institute.
DATE & VENUE
Tuesday, May 26 - Thursday, May 28, 2020
Fraunhofer Institute for Silicate Research
Translational Center for Regenerative Therapies
Röntgenring 11, 97070
Würzburg – GERMANY
PRICE
1310 EUR (1120 GBP)*
*Price excludes airfare and accomodations. Course will be charged in your region’s currency.
REGISTRATION DEADLINE
Extended to April 27, 2020
Space is limited to ensure personalized instruction. Please register early to guarantee your seat.
AGENDA
Reprogramming of Somatic Cells to Induced Pluripotent Stem Cells (iPS Cells)
Day 1
Session
Presenter/Trainer
9:00am - 9:30am
Welcome & Introductions
Fraunhofer and STEMCELL
9:30am - 10:30am
Lecture 1: Isolation and Reprogramming of Somatic Cells into iPS Cells
Dr. Katharina Debowski
10:30am - 10:45am
Break
10:45am - 12:00pm
Lecture 2: Maintaining and Assessing High-Quality hPSC Cultures
Raymond Lam
12:00pm - 1:00pm
Lunch (Courtesy of STEMCELL Technologies)
1:00pm - 2:00pm
Tour of the Stem Cell Facility
Fraunhofer
2:00pm - 3:00pm
Practical Session 1: Demonstration of Selection and Picking of iPS Cell Colonies
3:00pm - 3:15pm
Break
3:15pm - 4:15pm
Practical Session 2: Evaluate the Quality of hPSC Cultures and Plate Coating
- Observe time course of hPSC cultures to evaluate colony morphology
- Plate coating with mTeSR™1 & Matrigel® or TeSR™-E8™ & Vitronectin XF™
4:15pm - 4:30pm
Wrap Up
6:00pm
Course Dinner
Maintaining Human Pluripotent Stem Cells (hPSCs)
Day 2
Session
Presenter/Trainer
9:00am - 10:00am
Lecture 3: Cell Quality and Analysis Tools
Dr. Katharina Debowski
10:00am - 11:00am
Guest Lecture 1
Fraunhofer
11:00am - 12:00pm
Lunch (Courtesy of STEMCELL Technologies)
12:00pm - 2:00pm
Practical Session 3: Passaging hPSCs
- Demonstration and hands-on practice with enzyme-free passaging of hPSCs using Gentle Cell Dissociation Reagent (GCDR) and ReLeSR™
2:00pm - 2:15pm
Break
2:15pm - 3:15pm
Practical Session 4: Cryopreservation and Thawing of hPSCs
3:30pm - 4:30pm
Practical Session 5: Cell Quality Analysis
4:30pm - 4:45pm
Wrap Up Session
Differentiation of iPS Cells
Day 3
Session
Presenter/Trainer
9:00am - 9:30am
Lecture 4: Differentiation of iPSCs
Raymond Lam
9:30am - 12:00pm
Practical Session 6: Generate Embryoid Bodies (EBs) Using AggreWell™ and Prepare hPSCs as Single Cells for Monolayer-Based Protocol
12:00pm - 2:00pm
Lunch (Courtesy of STEMCELL Technologies)
1:00pm - 2:00pm
Guest Lecture 2
Fraunhofer
2:00pm - 4:00pm
Practical Session 7: Observation of Differentiated Cultures
4:00pm - 4:30pm
Wrap Up Session
- Q&A
- Quiz
- Course Evaluation
- Certificate Presentation
*Please note that the schedule is subject to change